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ZFIN ID: ZDB-FIG-101101-20
Kwon et al., 2009 - Mesendodermal signals required for otic induction: Bmp-antagonists cooperate with Fgf and can facilitate formation of ectopic otic tissue. Developmental dynamics : an official publication of the American Association of Anatomists   238(6):1582-1594 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 7 Patterning within medial otic vesicles. Expression of various markers in control embryos, or wild-type embryos treated with 70 μM SB431542 + 1 μM SU5402. A–F: Expression of AP markers pax5 (A, B), nkx5.1 (C, D), and fsta (E, F). G–L: Expression of DV markers dlx3b (G, H), otx1 (I, J), and ngn1 (K, L). M–P: Fluorescence/DIC overlays showing cranial ganglia in live embryos at 28 hpf, as visualized by islet2b:GFP. Neurons of the statoacoustic ganglia (sag) are indicated. Q, R: Bright field and fluorescence images of a live inhibitor-treated specimen at 30 hpf reveals the presence of sensory epithelia, marked by otoliths (Q, arrows) and brn3c:GFP expression in hair cells (R, arrows). Images show lateral views with anterior to the left (A–L, O, and P) or dorsal views with anterior to top (M, N, Q, R). Scale bar = 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT standard conditions Prim-5 otic vesicle ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 Prim-5 otic vesicle ISH
fsta WT standard conditions Prim-5 otic vesicle ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 Prim-5 Not Detected otic vesicle ISH
GFP s356tTg chemical treatment: SU5402, chemical treatment: SB 431542 Prim-15 hair cell IFL
zc7Tg standard conditions Prim-15 statoacoustic (VIII) ganglion IFL
zc7Tg chemical treatment: SU5402, chemical treatment: SB 431542 Prim-15 statoacoustic (VIII) ganglion IFL
hmx3a WT standard conditions Prim-5 otic vesicle ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 Prim-5 otic vesicle ISH
neurog1 WT standard conditions Prim-5 otic vesicle ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 Prim-5 otic vesicle ISH
otx1 WT standard conditions Prim-5 otic vesicle ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 Prim-5 otic vesicle ISH
pax5 WT standard conditions Prim-5 otic vesicle ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: SU5402, chemical treatment: SB 431542 Prim-5 otic vesicle formation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.