ZFIN ID: ZDB-FIG-101101-18
Kwon et al., 2009 - Mesendodermal signals required for otic induction: Bmp-antagonists cooperate with Fgf and can facilitate formation of ectopic otic tissue. Developmental dynamics : an official publication of the American Association of Anatomists   238(6):1582-1594 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Bud to 5-9 somites
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: 1-4 somites

Fig. 5 Paraxial cephalic mesoderm transfates to otic tissue in Nodal-Fgf-deficient embryos. A, B: Transverse section of a wild-type control stained for pax8 and shh. B shows an enlargement of the boxed region in A, with the mesodermal layer indicated (bracket). C, D: Transverse section through the otic domain of pax8 in a fgf8+/- heterozygote treated with 70 μM SB431542. D: An enlargement of the boxed area in C. E–H: Double staining for pax8 and fsta expression (dorsal views) in a wild-type control at 11.5 h (E), and wild-type embryos treated with 70 μM SB431542 + 1 μM SU5402 and fixed at 11 hpf (F) or 11.5 hpf (G, H). The normal otic domain of pax8 (ot) and somitic expression of fsta (som) are indicated. I, J: Two-color staining for fn1 (blue) and krox20 (red) at 10 hpf in a wild-type control (I) and a wild-type embryo treated with 70 μM SB431542 + 1 μM SU5402 (J). K, L: Double staining for pax2a and myoD in a wild-type control (K) and a wild-type embryo treated with 70 μM SB431542 + 1 μM SU5402 (L). The otic domain of pax2a (ot) and myoD-expressing somites (som) are indicated. M–T: Lineage-tracing of prospective mesendoderm. Laser irradiation was used to photo-uncage fluorescein-dextran in small clusters of marginal cells of wild-type embryos treated with 70 μM SB431542 + 1 μM SU5402 (M), and embryos were fixed and stained with anti-fluorescein antibody (blue) at 9.5 hpf (O), 13 hpf (P), or 24 hpf (T). Older specimens (P, T) were also stained by whole-mount in situ hybridization for pax2a expression (red). The specimen in T shows a sagittal section (anterior to left) of a medial otic vesicle containing scattered lineage-marked cells (arrows). For comparison, lineage-labeled cells are shown in wild-type controls at 9.5 hpf (N) and 13 hpf (Q), and at 13 hpf in fgf8+/- heterozygotes treated with 70 μM SB431542 (R) or fgf8+/- heterozygotes treated with 85 μM SB431542 (S). The specimen in R also shows krox20 expression (red). Whole-mount specimens show dorsal views with anterior to the top. Scale bar = 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT standard conditions Bud presumptive rhombomere 3 ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 Bud presumptive rhombomere 3 ISH
fn1a WT standard conditions Bud mesoderm ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 Bud mesoderm ISH
fsta WT standard conditions 1-4 somites somite ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 1-4 somites somite ISH
myod1 WT standard conditions 5-9 somites somite ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 5-9 somites somite ISH
pax2a WT standard conditions 5-9 somites otic placode ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 5-9 somites otic placode ISH
pax8 WT standard conditions 1-4 somites otic placode ISH
WT chemical treatment: SU5402, chemical treatment: SB 431542 1-4 somites otic placode ISH
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites otic placode ISH
1-4 somites paraxial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: SU5402, chemical treatment: SB 431542 1-4 somites otic placode formation process quality, abnormal
1-4 somites paraxial mesodermal cell fate specification process quality, abnormal
fgf8ati282a/+ chemical treatment: SB 431542 1-4 somites otic placode formation process quality, abnormal
1-4 somites paraxial mesodermal cell fate specification process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.