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ZFIN ID: ZDB-FIG-101101-15
Kwon et al., 2009 - Mesendodermal signals required for otic induction: Bmp-antagonists cooperate with Fgf and can facilitate formation of ectopic otic tissue. Developmental dynamics : an official publication of the American Association of Anatomists   238(6):1582-1594 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 1-4 somites to Prim-5

Fig. 2 Loss of Bmp-antagonists impairs otic induction. A, D: Expression of chd at 7.5 hpf, dorsal view (A), and chd and foxi1 at 9 hpf, lateral view (D). The preotic domain of foxi1 is indicated (ot). B, E: Expression of fsta at 10 hpf, dorsal view (B) and fsta and pax8, lateral view (E). The preotic domains of pax8 is indicated (ot). C, F: Expression of cv2 at 10.5 hpf as shown in a whole-mount specimen, dorsal view (C) and in cross section (F). The plane of section (f) and preplacodal ectoderm (ppe) are indicated. G–I: Otic vesicle at 26 hpf in a wild-type control (D), chd/fsta double-morphant (E), and chd/fsta/cv2 triple-morphant (F). J–L: Dorsal views of cldna expression in otic cells at 27 hpf in an fgf8-/- mutant (J), fgf8-/- mutant injected with chd/fsta-MO (K), and fgf8-/- mutant injected with chd/fsta/cv2-MO (L). M–R: Dorsal views of pax8 expression in pre-otic cells at 11 hpf (boxed area) in a wild-type control (M), chd/fsta double-morphant (N), chd/fsta/cv2 triple-morphant (O), fgf8-/- mutant (P), fgf8-/- mutant injected with chd/fsta-MO (Q), and fgf8-/- mutant injected with chd/fsta/cv2-MO (R). Anterior is to the top in all images except G–I in which anterior is to the left. Scale bar = 25 μm. S, schematic diagram of the fsta exon/intron structure, the fsta-MO2 target site (bar), primer-binding sites used for RT-PCR (P1, P2 arrows), and the results of RT-PCR confirming that fsta-MO2 injection severely reduces fsta transcript levels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmper WT standard conditions Bud paraxial mesoderm ISH
chrd WT standard conditions Shield presumptive dorsal mesoderm ISH
Shield presumptive paraxial mesoderm ISH
90%-epiboly axial mesoderm ISH
90%-epiboly presumptive paraxial mesoderm ISH
cldna fgf8ati282a/ti282a standard conditions Prim-5 otic vesicle ISH
fgf8ati282a/ti282a + MO1-chrd + MO2-bmper + MO6-fsta standard conditions Prim-5 otic vesicle ISH
fgf8ati282a/ti282a + MO1-chrd + MO6-fsta standard conditions Prim-5 otic vesicle ISH
foxi1 WT standard conditions 90%-epiboly non neural ectoderm ISH
fsta WT standard conditions Bud paraxial mesoderm ISH
pax8 WT standard conditions 1-4 somites otic placode ISH
Bud otic placode ISH
WT + MO1-chrd + MO2-bmper + MO6-fsta standard conditions 1-4 somites Not Detected otic placode ISH
WT + MO1-chrd + MO6-fsta standard conditions 1-4 somites otic placode ISH
fgf8ati282a/ti282a standard conditions 1-4 somites otic placode ISH
fgf8ati282a/ti282a + MO1-chrd + MO2-bmper + MO6-fsta standard conditions 1-4 somites Not Detected otic placode ISH
fgf8ati282a/ti282a + MO1-chrd + MO6-fsta standard conditions 1-4 somites otic placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-chrd + MO2-bmper + MO6-fsta standard conditions 1-4 somites otic placode formation process quality, abnormal
Prim-5 inner ear lacks all parts of type otolith, abnormal
Prim-5 otic vesicle decreased size, abnormal
Prim-5 otic vesicle formation process quality, abnormal
Prim-5 otic vesicle malformed, abnormal
WT + MO1-chrd + MO6-fsta standard conditions 1-4 somites otic placode formation process quality, abnormal
Prim-5 otic vesicle decreased size, abnormal
Prim-5 otic vesicle formation process quality, abnormal
fgf8ati282a/ti282a standard conditions 1-4 somites otic placode formation process quality, abnormal
fgf8ati282a/ti282a + MO1-chrd + MO2-bmper + MO6-fsta standard conditions 1-4 somites otic placode formation process quality, abnormal
Prim-5 otic vesicle aplastic, abnormal
Prim-5 otic vesicle formation process quality, abnormal
fgf8ati282a/ti282a + MO1-chrd + MO6-fsta standard conditions 1-4 somites otic placode formation process quality, abnormal
Prim-5 otic vesicle formation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.