ZFIN ID: ZDB-FIG-091215-22
Yue et al., 2009 - Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression. Cell   139(3):535-546 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Protruding-mouth

Fig. 3 β-Arrestin1 Critically Regulates Zebrafish Hematopoiesis

(A–F) Whole-mount double in situ hybridization for embryos at 13 hpf. Embryos injected with 4 ng control morpholino (Ctrl MO) or 4 ng β-arrestin1 morpholino (Arrb1 MO) were hybridized with riboprobes to early hematopoietic precursor marker scl (A and B), erythroid marker gata1 (C and D), and myeloid marker pu.1 (E and F). Embryos were flat-mounted with anterior to the left. Riboprobes to myoD was used to mark the somites for stage matching (red).

(G–N) Whole-mount in situ hybridization for embryos at 24 hpf. Embryos injected as (A-H) were hybridized with riboprobes to scl (G and H), hemoglobin marker hbbe3 (I and J), lmo2 (K and L), gata1 (M and N). Embryos are lateral views with anterior to the left. Arrows indicated the position of intermediate cell mass (ICM).

(O–R) Embryos derived from transgenic zebrafish lines Tg(lmo2:Rsred) (O and P) and Tg(gata1:EGFP) (Q and R) were injected with β-arrestin1 morpholino and showed significantly decreased levels of fluorescent intensity. Embryos are lateral views with anterior to the left.

(S–V) Depletion of β-arrestin1 displayed decreased hemoglobin staining by O-dianisidine at 36 hpf and 72 hpf (arrows).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a WT + MO1-arrb1 standard conditions 5-9 somites posterior lateral mesoderm ISH
Prim-5 Not Detected intermediate cell mass of mesoderm ISH
hbbe3 WT + MO1-arrb1 standard conditions Prim-5 Not Detected blood island ISH
Prim-5 intermediate cell mass of mesoderm ISH
lmo2 WT + MO1-arrb1 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
RFP rj2Tg + MO1-arrb1 standard conditions Prim-5 intermediate cell mass of mesoderm IFL
spi1b WT + MO1-arrb1 standard conditions 5-9 somites posterior lateral mesoderm ISH
tal1 WT + MO1-arrb1 standard conditions 5-9 somites posterior lateral mesoderm ISH
Prim-5 intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-arrb1 standard conditions Prim-25 nucleate erythrocyte decreased amount, abnormal
Prim-25 post-vent region curved ventral, abnormal
Prim-25 whole organism decreased length, abnormal
Protruding-mouth nucleate erythrocyte decreased amount, abnormal
Protruding-mouth post-vent region curved ventral, abnormal
Protruding-mouth whole organism decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 139(3), Yue, R., Kang, J., Zhao, C., Hu, W., Tang, Y., Liu, X., and Pei, G., Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression, 535-546, Copyright (2009) with permission from Elsevier. Full text @ Cell