ZFIN ID: ZDB-GENE-980526-164
Gene Name: Spi-1 proto-oncogene b
Gene Symbol: spi1b    Nomenclature History

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Previous Names: pu.1, pu1, spi1 (1), cb1086 (1), si:by184l24.2

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(Including Attributions)
Gene Type: protein_coding_gene
Location: Chr: 7 Mapping Details/Browsers
Description: Exhibits sequence-specific DNA binding activity. Involved in myeloid cell differentiation; negative regulation of myeloid cell differentiation; and positive regulation of gene expression. Predicted to localize to the nucleus. Is expressed in several structures, including hematopoietic cell; hematopoietic system; mesoderm; trunk; and yolk. Orthologous to human SPI1 (Spi-1 proto-oncogene).
Genome Resources: Alliance (1),  Gene:30117 (1),  Ensembl(GRCz11):ENSDARG00000000767 (2)
GENE EXPRESSION
All Expression Data: 182 figures from 120 publications
Directly Submitted Expression Data: 5 figures (10 images) from Thisse et al., 2001 [cb1086]
Wild-type Stages, Structures: Segmentation:1-4 somites (10.33h-11.66h) to Adult (90d-730d, breeding adult)
 
Curated Microarray Expression: GEO (1) , Expression Atlas (1)
MUTATIONS AND SEQUENCE TARGETING REAGENTS
Allele Type Localization Consequence Mutagen Suppliers
fh509 Small Deletion Exon 4 Unknown CRISPR
hkz4 Point Mutation Unknown Missense ENU
sa16105 Point Mutation Unknown Splice Site, Premature Stop ENU
  • European Zebrafish Resource Center (EZRC) (order this)
  • Zebrafish International Resource Center (ZIRC) (order this)
  • Sequence Targeting Reagents
    Targeting Reagent Created Alleles Publications
    CRISPR1-spi1b 1
    CRISPR2-spi1b
    2
    CRISPR3-spi1b
    2
    CRISPR4-spi1b
    2
    CRISPR5-spi1b
    2
    MO1-spi1b N/A 85
    MO2-spi1b N/A 20
    MO3-spi1b N/A 16
    MO4-spi1b N/A 1
    MO5-spi1b N/A 1
    MO6-spi1b N/A 3
    DISEASE ASSOCIATED WITH spi1b HUMAN ORTHOLOG No data available
    DISEASE ASSOCIATED WITH spi1b VIA EXPERIMENTAL MODELS No data available
    GENE ONTOLOGY
    Ontology GO Term
    Biological Process positive regulation of gene expression (more)
    Cellular Component nucleus (more)
    Molecular Function protein binding (more)
    GO Terms (all 16)
    PROTEIN FAMILIES, DOMAINS AND SITES
    Type InterPro ID Name
    Domain IPR000418 Ets domain
    Homologous_superfamily IPR036388 Winged helix-like DNA-binding domain superfamily
    Homologous_superfamily IPR036390 Winged helix DNA-binding domain superfamily
    TRANSCRIPTS
    Type Name Length (nt) Analysis
    mRNA spi1b-201 (1)    Ensembl 2373
    spi1b-202 (1)    Ensembl 1228
    ncRNA spi1b-003 (1)    Ensembl 688
    Browsers: UCSCNCBIEnsemblZFIN
    INTERACTIONS AND PATHWAYS
    ANTIBODIES
    Name Type Isotype Host Organism Assay Source Publications
    Ab2-spi1 Rabbit IHC 2
    Ab1-spi1 polyclonal IgG Rabbit Santa Cruz Biotechnology, Inc.
    2
    PLASMIDS No data available
    CONSTRUCTS WITH SEQUENCES FROM spi1b
    Construct Regulatory Regions Coding Sequences Species Tg Lines Publications
    Tg(-4spi1b:LY-EGFP) spi1b EGFPLY Danio rerio 1 3
    Tg(-5.3spi1b:EGFP) spi1b EGFP Danio rerio 1 7
    Tg(-9.0spi1b:EGFP) spi1b EGFP Danio rerio 1 27
    Tg(spi1b:GAL4,UAS:EGFP) spi1bUAS EGFPGAL4 Danio rerio 1 23
    Tg(spi1b:GAL4,UAS:LIFEACT-EGFP) spi1bUAS EGFPGAL4LIFEACT Danio rerio 1 1
    Tg(spi1b:GAL4,UAS:TagRFP) spi1b GAL4TagRFP Danio rerio 1 11
    Tg(spi1b:Hsa.FLT3-2A-EGFP,myl7:EGFP) myl7spi1b 2AEGFP Danio rerioHomo sapiens 1 2
    Tg(spi1b:Hsa.MYST3-ncoa2-EGFP) spi1b EGFP Danio rerioHomo sapiens 0 1
    Tg(spi1b:Hsa.NPM1,myl7:EGFP) myl7spi1b EGFP Danio rerioHomo sapiens 1 1
    Tg(spi1b:Hsa.SOCS1-2A-mCherry,myl7:EGFP) myl7spi1b 2AEGFPmCherry Danio rerioHomo sapiens 1 1
    Tg(spi1b:Hsa.SOX4-EGFP,myl7:EGFP) myl7spi1b EGFP Danio rerioHomo sapiens 1 1
    Tg(spi1b:LOXP-EGFP-LOXP-Hsa.NUP98-Hsa.HOXA9) spi1b EGFP Danio rerioHomo sapiens 1 4
    MARKER RELATIONSHIPS
    spi1b Contained in: [BAC] CH73-314G15 (1), CH211-150G13 (1), XX-184L24
    spi1b Encodes: [EST] cb1086 (1) (order this)
    [cDNA] MGC:65932 (1), MGC:85783 (1)
    SEQUENCE INFORMATION
    Type Accession # Length (nt/aa) Analysis
    RNA RefSeq:NM_198062 (1) 1034 nt
    Genomic GenBank:AL929252 (1) 173244 nt
    Select Tool
    Polypeptide UniProtKB:Q6WEE1 (1) 297 aa
    Sequence Information (all 36)
    ORTHOLOGY for spi1b ( Chr: 7 )
    CITATIONS (339)