PUBLICATION
Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression
- Authors
- Yue, R., Kang, J., Zhao, C., Hu, W., Tang, Y., Liu, X., and Pei, G.
- ID
- ZDB-PUB-091120-6
- Date
- 2009
- Source
- Cell 139(3): 535-546 (Journal)
- Registered Authors
- Keywords
- DEVBIO, DNA, PROTEINS
- Datasets
- GEO:GSE17773
- MeSH Terms
-
- Animals
- Hematopoiesis*
- Genes, Homeobox
- Polycomb-Group Proteins
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- Signal Transduction*
- Homeodomain Proteins/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- YY1 Transcription Factor/metabolism*
- Repressor Proteins/metabolism*
- Arrestins/metabolism*
- PubMed
- 19879840 Full text @ Cell
Citation
Yue, R., Kang, J., Zhao, C., Hu, W., Tang, Y., Liu, X., and Pei, G. (2009) Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression. Cell. 139(3):535-546.
Abstract
Beta-arrestin1 is a multifunctional protein critically involved in signal transduction. Recently, it is also identified as a nuclear transcriptional regulator, but the underlying mechanisms and physiological significance remain to be explored. Here, we identified beta-arrestin1 as an evolutionarily conserved protein essential for zebrafish development. Zebrafish embryos depleted of beta-arrestin1 displayed severe posterior defects and especially failed to undergo hematopoiesis. In addition, the expression of cdx4, a critical regulator of embryonic blood formation, and its downstream hox genes were downregulated by depletion of beta-arrestin1, while injection of cdx4, hoxa9a or hoxb4a mRNA rescued the hematopoietic defects. Further mechanistic studies revealed that beta-arrestin1 bound to and sequestered the polycomb group (PcG) recruiter YY1, and relieved PcG-mediated repression of cdx4-hox pathway, thus regulating hematopoietic lineage specification. Taken together, this study demonstrated a critical role of beta-arrestin1 during zebrafish primitive hematopoiesis, as well as an important regulator of PcG proteins and cdx4-hox pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping