FIGURE

Fig. 5

ID

ZDB-FIG-071116-4

Publication

Wingert et al., 2007 - The cdx Genes and Retinoic Acid Control the Positioning and Segmentation of the Zebrafish Pronephros

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Fig. 5

cdx Genes Position the Pronephros along the Embryonic Axis and Are Requisite for Distal Segment Formation. Expression of segment markers was performed on wild-type, cdx4^–/– mutants, and cdx-deficient embryos at (A) 26 hpf and (B) 48 hpf using whole-mount in situ hybridization. Brackets and arrows indicate expression domains in each embryo. Numbers indicate somite number. Dorsal views of embryos, with anterior to the left, with the exception of lateral views for gata3 and aqp3 expression. (C) Live wild-type, cdx4^–/– mutants, and cdx-deficient embryos at 72 hpf. Arrowhead indicates glomerular cyst in the cdx-deficient embryo. Lateral views are shown, with anterior to the left. (D) Summary of nephron segmentation in cdx4^–/– mutants and cdx-deficient embryos as compared to wild-type at 48 hpf. Numbers indicate anterior somite boundary of each pronephros.

Expression Data

Genes:	aqp3a cdh17 gata3 mafba slc12a1 slc12a3 slc20a1a stc1l trpm7 wt1a wt1b
Fish:	TU cdx4^{tv205c/tv205c} (TU) cdx4^{tv205c/tv205c} + MO2-cdx1a (TU)
Knockdown Reagent:	MO2-cdx1a
Anatomical Terms:	cloacal chamber corpuscles of Stannius podocyte posterior pronephric duct pronephric distal early tubule pronephric distal late tubule pronephric proximal convoluted tubule pronephric proximal straight tubule pronephric tubule trunk mesenchyme
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdx4^{tv205c/tv205c} (TU) cdx4^{tv205c/tv205c} + MO2-cdx1a (TU)
Knockdown Reagent:	MO2-cdx1a
Observed In:	corpuscles of Stannius pericardium podocyte posterior pronephric duct pronephric distal early tubule pronephric distal late tubule pronephric duct opening pronephric glomerulus pronephric podocyte pronephric proximal convoluted tubule pronephric proximal straight tubule trunk mesenchyme
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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