ZFIN ID: ZDB-FIG-071116-3
Wingert et al., 2007 - The cdx Genes and Retinoic Acid Control the Positioning and Segmentation of the Zebrafish Pronephros. PLoS Genetics   3(10):1922-1938 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 4 Exogenous RA Treatment Proximalizes the Pronephros. (A) Expression of segment markers and mhc at 24 hpf in wild-type embryos incubated with either DMSO (control) or RA at the indicated dosage and times during development, by double whole-mount in situ hybridization. Brackets and arrows indicate expression domains. Abbreviations are: %e (percent epiboly), s (somite stage). Dorsal views are shown, with anterior to the left, with the exception of lateral views for gata3 expression. (B) Summary of nephron segmentation with respect to embryo somite number for each RA experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdh17 TU control Prim-5 posterior pronephric duct ISH
Prim-5 pronephric distal early tubule ISH
Prim-5 pronephric distal late tubule ISH
Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
Prim-5 pronephric tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 posterior pronephric duct ISH
Prim-5 pronephric distal early tubule ISH
Prim-5 pronephric distal late tubule ISH
Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
Prim-5 pronephric tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 posterior pronephric duct ISH
Prim-5 pronephric distal early tubule ISH
Prim-5 pronephric distal late tubule ISH
Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
Prim-5 pronephric tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 posterior pronephric duct ISH
Prim-5 pronephric distal early tubule ISH
Prim-5 pronephric distal late tubule ISH
Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
Prim-5 pronephric tubule ISH
gata3 TU control Prim-5 posterior pronephric duct ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 posterior pronephric duct ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 Not Detected posterior pronephric duct ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 posterior pronephric duct ISH
ret TU control Prim-5 pronephric distal late tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric distal late tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 Not Detected pronephric distal late tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric distal late tubule ISH
slc9a3r1a TU control Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
slc12a1 TU control Prim-5 pronephric distal early tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric distal early tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 Not Detected pronephric distal early tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric distal early tubule ISH
slc20a1a TU control Prim-5 pronephric proximal convoluted tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal convoluted tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal convoluted tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal convoluted tubule ISH
stc1l TU control Prim-5 corpuscles of Stannius ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 corpuscles of Stannius ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 Not Detected corpuscles of Stannius ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 corpuscles of Stannius ISH
trpm7 TU control Prim-5 pronephric proximal straight tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal straight tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal straight tubule ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric proximal straight tubule ISH
wt1b TU control Prim-5 podocyte ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 podocyte ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 podocyte ISH
TU chemical treatment: all-trans-retinoic acid Prim-5 podocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric distal early tubule decreased length, abnormal
Prim-5 pronephric distal late tubule decreased length, abnormal
Prim-5 pronephric proximal convoluted tubule increased length, abnormal
Prim-5 pronephric proximal straight tubule increased length, abnormal
TU chemical treatment: all-trans-retinoic acid Prim-5 posterior pronephric duct aplastic, abnormal
Prim-5 pronephric distal early tubule aplastic, abnormal
Prim-5 pronephric distal late tubule aplastic, abnormal
Prim-5 pronephric proximal convoluted tubule increased length, abnormal
Prim-5 pronephric proximal straight tubule increased length, abnormal
TU chemical treatment: all-trans-retinoic acid Prim-5 pronephric distal early tubule decreased length, abnormal
Prim-5 pronephric distal late tubule decreased length, abnormal
Prim-5 pronephric proximal convoluted tubule increased length, abnormal
Prim-5 pronephric proximal straight tubule increased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.