ZFIN ID: ZDB-FIG-190723-887
Zhang et al., 2019 - Endoglin is a conserved regulator of vasculogenesis in zebrafish-Implications for hereditary haemorrhagic telangiectasia. Bioscience Reports   39(5) Full text @ Biosci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Figure 4

Endoglin knockdown decreased the expression of endothelial markers

(A) RT-PCR for endothelial markers in the Con-MO and eng-MO group, including kdrl, cdh5, hey2, lmo2 and pu.1 (A). Gapdh was used as an internal control. (B) Quantification of mRNA expression in (A) with grey scanning analysis, conducted with ImageJ. (C) WISH for endothelial marker expression in the Con-MO and eng-MO group among 24 hpf embryos (n>30 for each group). The red arrow indicates the region where the endothelial markers were significantly decreased. Scale bars are 200 μm. (D) qPCR for cdh5, dll4, flt4 and kdrl expression in the brain and trunk of the Con-MO and eng-MO group. Gapdh was used as an internal control (error bars, SEM; n=3 biological replicates). A value of P was considered statistically significant (*P<0.05, **P<0.01, ***P<0.001) for B and D.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 WT control Prim-5 brain RTPCR
Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
Prim-5 trunk RTPCR
Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 brain RTPCR
Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
Prim-5 trunk RTPCR
Prim-5 whole organism RTPCR
dll4 WT control Prim-5 brain RTPCR
Prim-5 trunk RTPCR
WT + MO1-eng standard conditions Prim-5 brain RTPCR
Prim-5 trunk RTPCR
flt4 WT control Prim-5 brain RTPCR
Prim-5 trunk RTPCR
WT + MO1-eng standard conditions Prim-5 brain RTPCR
Prim-5 trunk RTPCR
hey2 WT control Prim-5 endothelial cell ISH
WT standard conditions Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 endothelial cell ISH
Prim-5 whole organism RTPCR
kdrl WT control Prim-5 brain RTPCR
Prim-5 endothelial cell ISH
Prim-5 trunk RTPCR
Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 brain RTPCR
Prim-5 endothelial cell ISH
Prim-5 trunk RTPCR
Prim-5 whole organism RTPCR
lmo2 WT control Prim-5 endothelial cell ISH
WT standard conditions Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 endothelial cell ISH
Prim-5 whole organism RTPCR
spi1b WT control Prim-5 endothelial cell ISH
WT standard conditions Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 endothelial cell ISH
Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-eng standard conditions Prim-5 brain cdh5 expression decreased amount, abnormal
Prim-5 brain flt4 expression decreased amount, abnormal
Prim-5 brain kdrl expression decreased amount, abnormal
Prim-5 dorsal aorta cdh5 expression decreased distribution, abnormal
Prim-5 endothelial cell hey2 expression decreased distribution, abnormal
Prim-5 endothelial cell kdrl expression decreased distribution, abnormal
Prim-5 endothelial cell lmo2 expression decreased distribution, abnormal
Prim-5 endothelial cell spi1b expression decreased distribution, abnormal
Prim-5 posterior cardinal vein cdh5 expression decreased distribution, abnormal
Prim-5 trunk cdh5 expression decreased amount, abnormal
Prim-5 trunk dll4 expression decreased amount, abnormal
Prim-5 trunk flt4 expression decreased amount, abnormal
Prim-5 trunk kdrl expression decreased amount, abnormal
Prim-5 whole organism cdh5 expression decreased amount, abnormal
Prim-5 whole organism hey2 expression decreased amount, abnormal
Prim-5 whole organism kdrl expression decreased amount, abnormal
Prim-5 whole organism lmo2 expression decreased amount, abnormal
Prim-5 whole organism spi1b expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biosci. Rep.