ZFIN ID: ZDB-FIG-190723-888
Zhang et al., 2019 - Endoglin is a conserved regulator of vasculogenesis in zebrafish-Implications for hereditary haemorrhagic telangiectasia. Bioscience Reports   39(5) Full text @ Biosci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 64-cell to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 75%-epiboly to Long-pec

Figure 5

Bmper was involved in endoglin-regulated vasculogenesis

(A) Heat maps presenting part of the RNA-Seq result. Six vasculogenesis genes were significantly decreased after endoglin knockdown, including bmper, kmt2e, use1, csnk2a1, tmem199 and sin3aa. The level of expression of the gene is indicated with red (high) or blue (low). (B) qPCR analysis of bmper expression in different stages of the Con-MO and eng-MO group, including 64 cell, sphere, 30% epiboly, 75% epiboly, 11 hpf and 24 hpf stage. Gapdh was used as an internal control (mean ± SD; the experiments were repeated three times). (C) Bmper expression by WISH in 24 hpf zebrafish embryos of the Con-MO and eng-MO group. The red arrow indicates the region where the expression of bmper was significantly decreased. (D) Fluorescence image of Con-MO, Con-MO + bmper mRNA, eng-MO and eng-MO + bmper mRNA group. (All Tg(fli1a:EGFP)y1 zebrafish embryos were injected with 2 ng morpholinos and 500 pg mRNA. For example, Con-MO group: 2 ng 5-mispair control MO and 500 pg mCherry mRNA.) The red and white arrows point to the disrupted and rescued structure of DLAV and ISVs, respectively. (E) qPCR analysis for cdh5, dll4, flt4 and kdrl expression in fli-GFP+ cells of the Con-MO, eng-MO and eng-MO + bmper mRNA group. Gapdh was used as an internal control (mean ± SD; the experiments were repeated three times). A value of P was considered statistically significant (*P<0.05) for B and E. Scale bars are 100 μm for C and D.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmper WT control 64-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
75%-epiboly whole organism RTPCR
1-4 somites whole organism RTPCR
Prim-5 blood island ISH
Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions 64-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
75%-epiboly whole organism RTPCR
1-4 somites whole organism RTPCR
Prim-5 blood island ISH
Prim-5 whole organism RTPCR
cdh5 WT control Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 whole organism RTPCR
dll4 WT control Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 whole organism RTPCR
EGFP y1Tg control Prim-5 vasculature IFL
Long-pec intersegmental vein IFL
y1Tg + MO1-eng standard conditions Prim-5 vasculature IFL
Long-pec intersegmental vein IFL
flt4 WT control Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 whole organism RTPCR
kdrl WT control Prim-5 whole organism RTPCR
WT + MO1-eng standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-eng standard conditions 75%-epiboly whole organism bmper expression decreased amount, abnormal
1-4 somites whole organism bmper expression decreased amount, abnormal
Prim-5 blood island bmper expression decreased distribution, abnormal
Prim-5 whole organism bmper expression decreased amount, abnormal
Prim-5 whole organism cdh5 expression decreased amount, abnormal
Prim-5 whole organism dll4 expression decreased amount, abnormal
Prim-5 whole organism flt4 expression decreased amount, abnormal
Prim-5 whole organism kdrl expression decreased amount, abnormal
y1Tg + MO1-eng standard conditions Prim-5 vasculature EGFP expression decreased distribution, abnormal
Long-pec intersegmental vein EGFP expression decreased distribution, abnormal
Acknowledgments:
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