ZFIN ID: ZDB-FIG-151120-12
Won et al., 2015 - Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas. Proceedings of the National Academy of Sciences of the United States of America   112(40):12426-31 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Pec-fin

Fig. 2

lnx2a knockdown causes defects in the exocrine pancreas. (A) Schematic drawing of the lnx2a locus containing 11 exons, and the Lnx2a protein (737 aa) containing a RING-finger domain (RF), the Numb binding NPAF motif (F), and four PDZ domains. The translation initiation site is located in exon 2, which encodes the RING-finger domain. The lnx2a-MO targets the splice donor site of exon 2. Primers in exon 1 (F1) and exon 11 (R1) are shown, as is the epitope for the Lnx2a antibody in the third PDZ domain. (B and C) The lnx2a-MO (5 ng) leads to inhibition of exocrine markers (try, ptf1a), but not endocrine markers (ins, glu), as seen by two-color WISH at 60 hpf. (D and E) Quantification of marker expression; effects were classified as strong, partial, and unaffected. MO, lnx2a-MO; UN, uninjected. (Scale bar: 100 ┬Ám.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga AB control Pec-fin endocrine pancreas ISH
AB + MO1-lnx2a control Pec-fin endocrine pancreas ISH
ins AB control Pec-fin endocrine pancreas ISH
AB + MO1-lnx2a control Pec-fin endocrine pancreas ISH
prss1 AB control Pec-fin exocrine pancreas ISH
AB + MO1-lnx2a control Pec-fin exocrine pancreas ISH
ptf1a AB control Pec-fin exocrine pancreas ISH
AB + MO1-lnx2a control Pec-fin exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-lnx2a control Pec-fin exocrine pancreas prss1 expression decreased amount, abnormal
Pec-fin exocrine pancreas ptf1a expression decreased amount, abnormal
Pec-fin exocrine pancreas ptf1a expression decreased distribution, abnormal
Pec-fin exocrine pancreas prss1 expression decreased distribution, abnormal
Pec-fin exocrine pancreas development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA