ZFIN ID: ZDB-FIG-160330-2
Won et al., 2015 - Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas. Proceedings of the National Academy of Sciences of the United States of America   112(40):12426-31 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: High-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: High-pec

Fig. 7

lnx2a-MO injection results in impaired cell proliferation in the ventral pancreas. (A) Tg(ptf1a:EGFP) embryos were incubated in 10 mM BrdU from 46 to 47 hpf, fixed, stained with anti-BrdU and anti-Numb antibodies, and analyzed by confocal microscopy. For clarity, the same plane of the BrdU channel is shown separately in addition to the merged images. (Scale bar: 10 µm.) (B) The average number of ptf1:EGFP+, Numb+ and ptf1:EGFP+/BrdU+ double-positive cells in the pancreas of 10 uninjected and lnx2a-MO–injected embryos are shown (***P < 0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP jh1Tg control High-pec anterior pancreatic bud IFL
jh1Tg + MO1-lnx2a control High-pec anterior pancreatic bud IFL
numb jh1Tg control High-pec pancreatic bud IHC
jh1Tg + MO1-lnx2a control High-pec pancreatic bud IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jh1Tg control High-pec pancreatic bud numb expression increased distribution, abnormal
jh1Tg + MO1-lnx2a control High-pec anterior pancreatic bud EGFP expression decreased distribution, abnormal
High-pec anterior pancreatic bud cell population proliferation decreased process quality, abnormal
High-pec pancreatic bud numb expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA