|ZFIN ID: ZDB-FIG-160330-2|
lnx2a-MO injection results in impaired cell proliferation in the ventral pancreas. (A) Tg(ptf1a:EGFP) embryos were incubated in 10 mM BrdU from 46 to 47 hpf, fixed, stained with anti-BrdU and anti-Numb antibodies, and analyzed by confocal microscopy. For clarity, the same plane of the BrdU channel is shown separately in addition to the merged images. (Scale bar: 10 µm.) (B) The average number of ptf1:EGFP+, Numb+ and ptf1:EGFP+/BrdU+ double-positive cells in the pancreas of 10 uninjected and lnx2a-MO–injected embryos are shown (***P < 0.001).
|EGFP||jh1Tg||control||High-pec||anterior pancreatic bud||IFL|
|jh1Tg + MO1-lnx2a||control||High-pec||anterior pancreatic bud||IFL|
|jh1Tg + MO1-lnx2a||control||High-pec||pancreatic bud||IHC|
|Acknowledgments:||ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA|