Ventral pancreas defects in lnx2aΔ329 mutant and lnx2a-MO–injected embryos can be rescued by knockdown of Numb. (A) The translation blocking MO for Numb (5 ng) was injected into WT, lnx2aΔ329-, and lnx2a-MO–injected embryos, and pancreatic phenotype was examined at 60 hpf. (Scale bar: 50 µm.) (B) Quantification of pancreatic defects by analysis of ptf1a expression. Exocrine pancreas defects were substantially rescued by knockdown of Numb; numb-MO had little effect in WT embryos. (C) Immunoblotting of embryo extracts with Numb antibody shows the efficiency of the numb-MO.