ZFIN ID: ZDB-FIG-151120-15
Won et al., 2015 - Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas. Proceedings of the National Academy of Sciences of the United States of America   112(40):12426-31 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Pec-fin

Fig. 5

Ventral pancreas defects in lnx2aΔ329 mutant and lnx2a-MO–injected embryos can be rescued by knockdown of Numb. (A) The translation blocking MO for Numb (5 ng) was injected into WT, lnx2aΔ329-, and lnx2a-MO–injected embryos, and pancreatic phenotype was examined at 60 hpf. (Scale bar: 50 µm.) (B) Quantification of pancreatic defects by analysis of ptf1a expression. Exocrine pancreas defects were substantially rescued by knockdown of Numb; numb-MO had little effect in WT embryos. (C) Immunoblotting of embryo extracts with Numb antibody shows the efficiency of the numb-MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga AB control Pec-fin endocrine pancreas ISH
AB + MO1-lnx2a control Pec-fin endocrine pancreas ISH
AB + MO1-lnx2a + MO4-numb control Pec-fin endocrine pancreas ISH
AB + MO4-numb control Pec-fin endocrine pancreas ISH
lnx2ay447/y447 control Pec-fin endocrine pancreas ISH
lnx2ay447/y447 + MO4-numb control Pec-fin endocrine pancreas ISH
ins AB control Pec-fin endocrine pancreas ISH
AB + MO1-lnx2a control Pec-fin endocrine pancreas ISH
AB + MO1-lnx2a + MO4-numb control Pec-fin endocrine pancreas ISH
AB + MO4-numb control Pec-fin endocrine pancreas ISH
lnx2ay447/y447 control Pec-fin endocrine pancreas ISH
lnx2ay447/y447 + MO4-numb control Pec-fin endocrine pancreas ISH
prss1 AB control Pec-fin exocrine pancreas ISH
AB + MO1-lnx2a control Pec-fin exocrine pancreas ISH
AB + MO1-lnx2a + MO4-numb control Pec-fin exocrine pancreas ISH
AB + MO4-numb control Pec-fin exocrine pancreas ISH
lnx2ay447/y447 control Pec-fin exocrine pancreas ISH
lnx2ay447/y447 + MO4-numb control Pec-fin exocrine pancreas ISH
ptf1a AB control Pec-fin exocrine pancreas ISH
AB + MO1-lnx2a control Pec-fin exocrine pancreas ISH
AB + MO1-lnx2a + MO4-numb control Pec-fin exocrine pancreas ISH
AB + MO4-numb control Pec-fin exocrine pancreas ISH
lnx2ay447/y447 control Pec-fin exocrine pancreas ISH
lnx2ay447/y447 + MO4-numb control Pec-fin exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-lnx2a control Pec-fin exocrine pancreas prss1 expression decreased amount, abnormal
Pec-fin exocrine pancreas ptf1a expression decreased amount, abnormal
Pec-fin exocrine pancreas prss1 expression decreased distribution, abnormal
Pec-fin exocrine pancreas ptf1a expression decreased distribution, abnormal
Pec-fin exocrine pancreas development disrupted, abnormal
AB + MO1-lnx2a + MO4-numb control Pec-fin exocrine pancreas ptf1a expression amount, ameliorated
Pec-fin exocrine pancreas prss1 expression amount, ameliorated
Pec-fin exocrine pancreas ptf1a expression spatial pattern, ameliorated
Pec-fin exocrine pancreas prss1 expression spatial pattern, ameliorated
Pec-fin exocrine pancreas development process quality, ameliorated
lnx2ay447/y447 control Pec-fin exocrine pancreas ptf1a expression decreased amount, abnormal
Pec-fin exocrine pancreas prss1 expression decreased amount, abnormal
Pec-fin exocrine pancreas prss1 expression decreased distribution, abnormal
Pec-fin exocrine pancreas ptf1a expression decreased distribution, abnormal
Pec-fin exocrine pancreas development disrupted, abnormal
lnx2ay447/y447 + MO4-numb control Pec-fin exocrine pancreas prss1 expression amount, ameliorated
Pec-fin exocrine pancreas ptf1a expression amount, ameliorated
Pec-fin exocrine pancreas ptf1a expression spatial pattern, ameliorated
Pec-fin exocrine pancreas prss1 expression spatial pattern, ameliorated
Pec-fin exocrine pancreas development process quality, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA