| ZFIN ID: ZDB-FIG-160330-1 |
Won et al., 2015
- Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas.
Proceedings of the National Academy of Sciences of the United States of America
112(40):12426-31
Full text @ Proc. Natl. Acad. Sci. USA
EXPRESSION / LABELING:
| Genes: | |
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| Fish: | |
| Knockdown Reagents: | |
| Anatomical Term: | |
| Stage: | Long-pec |
PHENOTYPE:
| Fish: | |
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| Knockdown Reagents: | |
| Observed In: | |
| Stage: | Long-pec |
Fig. 6 lnx2a-MO injection causes a reduction of Notch-responsive cells in the pancreas. (A) Tg(Tp1:H2BmCherry); Tg(Tp1:VenusPest) embryos were injected with lnx2a-MO (A2), numb-MO (A3), or both (A4). At 48 hpf, embryos were stained with anti-Numb antibody and analyzed by confocal microscopy; Z projections are shown. (Scale bar: 10 µm.) (B) Total number of Notch-OFF (Tp1:H2BmCherry+) and Notch-ON (Tp1:H2BmCherry+ and Tp1:VenusPest+) cells, as well as Numb-positive cells, were counted in all Z sections of four embryos for each category. **P < 0.005; ***P < 0.002. |
Gene Expression Details
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| mCherry | s939Tg; s940Tg | control | Long-pec | pancreas | IFL | |
| s939Tg; s940Tg + MO1-lnx2a | control | Long-pec | pancreas | IFL | ||
| s939Tg; s940Tg + MO1-lnx2a + MO4-numb | control | Long-pec | pancreas | IFL | ||
| s939Tg; s940Tg + MO4-numb | control | Long-pec | pancreas | IFL | ||
| numb | s939Tg; s940Tg | control | Long-pec | pancreas | IHC | |
| s939Tg; s940Tg + MO1-lnx2a | control | Long-pec | pancreas | IHC | ||
| s939Tg; s940Tg + MO1-lnx2a + MO4-numb | control | Long-pec | pancreas | IHC | ||
| s939Tg; s940Tg + MO4-numb | control | Long-pec | pancreas | IHC | ||
| Venus | s939Tg; s940Tg | control | Long-pec | pancreas | IFL | |
| s939Tg; s940Tg + MO1-lnx2a | control | Long-pec | pancreas | IFL | ||
| s939Tg; s940Tg + MO1-lnx2a + MO4-numb | control | Long-pec | pancreas | IFL | ||
| s939Tg; s940Tg + MO4-numb | control | Long-pec | pancreas | IFL |
Antibody Labeling Details
No data available
Phenotype Details
| Acknowledgments: | ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA |
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