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ZFIN ID: ZDB-FIG-160330-1
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Won et al., 2015 - Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas. Proc. Natl. Acad. Sci. USA   112(40):12426-31 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 6

lnx2a-MO injection causes a reduction of Notch-responsive cells in the pancreas. (A) Tg(Tp1:H2BmCherry); Tg(Tp1:VenusPest) embryos were injected with lnx2a-MO (A2), numb-MO (A3), or both (A4). At 48 hpf, embryos were stained with anti-Numb antibody and analyzed by confocal microscopy; Z projections are shown. (Scale bar: 10 µm.) (B) Total number of Notch-OFF (Tp1:H2BmCherry+) and Notch-ON (Tp1:H2BmCherry+ and Tp1:VenusPest+) cells, as well as Numb-positive cells, were counted in all Z sections of four embryos for each category. **P < 0.005; ***P < 0.002.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry s939Tg; s940Tg control Long-pec pancreas IFL
s939Tg; s940Tg + MO1-lnx2a control Long-pec pancreas IFL
s939Tg; s940Tg + MO1-lnx2a + MO4-numb control Long-pec pancreas IFL
s939Tg; s940Tg + MO4-numb control Long-pec pancreas IFL
numb s939Tg; s940Tg control Long-pec pancreas IHC
s939Tg; s940Tg + MO1-lnx2a control Long-pec pancreas IHC
s939Tg; s940Tg + MO1-lnx2a + MO4-numb control Long-pec pancreas IHC
s939Tg; s940Tg + MO4-numb control Long-pec pancreas IHC
Venus s939Tg; s940Tg control Long-pec pancreas IFL
s939Tg; s940Tg + MO1-lnx2a control Long-pec pancreas IFL
s939Tg; s940Tg + MO1-lnx2a + MO4-numb control Long-pec pancreas IFL
s939Tg; s940Tg + MO4-numb control Long-pec pancreas IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s939Tg; s940Tg + MO1-lnx2a control Long-pec pancreas mCherry expression decreased distribution, abnormal
Long-pec pancreas Venus expression decreased distribution, abnormal
Long-pec pancreas numb expression increased distribution, abnormal
s939Tg; s940Tg + MO1-lnx2a + MO4-numb control Long-pec pancreas numb expression spatial pattern, ameliorated
s939Tg; s940Tg + MO1-lnx2a + MO4-numb control Long-pec pancreas Venus expression spatial pattern, ameliorated
s939Tg; s940Tg + MO1-lnx2a + MO4-numb control Long-pec pancreas mCherry expression spatial pattern, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA