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ZFIN ID: ZDB-FIG-150708-18
Butko et al., 2015 - Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo. Development (Cambridge, England)   142:1050-61 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 10-13 somites to Prim-25

Fig. S4 gata2b morphants have normal primitive hematopoiesis, morphogenesis, and gata2a expression. (A-R) Whole mount in situ hybridization of wildtype embryos and gata2b morphant siblings. Expression of etsrp at 10 ss (A, B). (C-H) Expression of gata1a (C, D), scl (E, F), and runx1 (G, H) in 12 ss embryos. (I-N) Expression of gata1a (I,J), scl (K, L) and l-plastin (M, N) in 22 hpf embryos. (O, P) Expression of myod in 25 hpf embryos. (Q, R) Expression of cdh17 in 36 hpf embryos. (S, T) Expression of gata2a in 25 hpf control (S) and gata2b morphant embryos (T).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh17 WT control Prim-25 pronephric duct ISH
WT + MO1-gata2b standard conditions Prim-25 pronephric duct ISH
etsrp WT control 10-13 somites posterior lateral mesoderm ISH
WT + MO1-gata2b standard conditions 10-13 somites posterior lateral mesoderm ISH
gata1a WT control 10-13 somites posterior lateral mesoderm ISH
26+ somites erythroid lineage cell ISH
WT + MO1-gata2b standard conditions 10-13 somites posterior lateral mesoderm ISH
26+ somites erythroid lineage cell ISH
gata2b WT control Prim-5 ventral wall of dorsal aorta ISH
WT + MO1-gata2b standard conditions Prim-5 ventral wall of dorsal aorta ISH
lcp1 WT control 26+ somites macrophage ISH
WT + MO1-gata2b standard conditions 26+ somites macrophage ISH
myod1 WT control Prim-5 somite ISH
WT + MO1-gata2b standard conditions Prim-5 somite ISH
runx1 WT control 10-13 somites posterior lateral mesoderm ISH
WT + MO1-gata2b standard conditions 10-13 somites posterior lateral mesoderm ISH
tal1 WT control 10-13 somites posterior lateral mesoderm ISH
26+ somites erythroid lineage cell ISH
WT + MO1-gata2b standard conditions 10-13 somites posterior lateral mesoderm ISH
26+ somites erythroid lineage cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gata2b standard conditions 10-13 somites posterior lateral mesoderm physical object quality, normal
26+ somites primitive hemopoiesis process quality, normal
Prim-5 somite development process quality, normal
Prim-25 pronephric duct development process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development