header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150708-15
Butko et al., 2015 - Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo. Development (Cambridge, England)   142:1050-61 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. S1

gata2a is expressed within the vasculature, and is required for vascular integrity. (A) In situ hybridization of gata2a expression in the PLM at the 8-somite stage (13 hpf) (B) Expression of gata2a at 25 hpf. (C) Confocal microscopy shows a lateral view of the trunk region of gata2a:GFP;kdrl:mCherrymem at 25 hpf. (C′) Digitally reconstructed transverse view of the trunk region in (C). (D-E) Pooling of red blood cells observed by o-dianisidine staining in 2 dpf siblings (D) and gata2aum27 mutants (E). (F) Confocal microscopy of the head vasculature and erythrocytes in a 2 dpf lmo2:GFP;gata1a:DsRed;gata2aum27-/- embryo. White arrows denote extravascular erythrocytes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed gata2aum27/um27; sd2Tg; zf72Tg control Long-pec nucleate erythrocyte IFL
EGFP gata2aum27/um27; sd2Tg; zf72Tg control Long-pec cranial vasculature IFL
la3Tg; s896Tg control Prim-5 vascular endothelium IFL
gata2a WT control 5-9 somites posterior lateral mesoderm ISH
Prim-5 vascular endothelium ISH
mCherry la3Tg; s896Tg control Prim-5 vascular endothelium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gata2aum27/um27 control Long-pec nucleate erythrocyte accumulation trunk, abnormal
gata2aum27/um27; sd2Tg; zf72Tg control Long-pec cranial vasculature broken, abnormal
Long-pec nucleate erythrocyte accumulation head, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development