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General Information
ZIRC
ZFIN ID: ZDB-FIG-150422-45
Butko et al., 2015 - Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo. Development (Cambridge, England)   142:1050-61 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 3

Tg(gata2b:Gal4) marks hemogenic endothelial cells. Imaging is by confocal microscopy. (A) GFP+ endothelial cells in the trunk region of a gata2b:Gal4;UAS:GFP embryo at 24hpf. (B-C′) gata2b:Gal4;UAS:lifeactGFP at 30hpf and 3dpf. (C′) An enlarged view of C, with thymus and kidney highlighted. (D,D′) Transverse (D) and lateral (D′) views of the trunk vasculature of 30hpf gata2b:Gal4;UAS:GFP;kdrl:mCherrymem. (E,E′) Transverse (E) and lateral (E′) views of a 48hpf gata2b:Gal4;UAS:GFP;kdrl:Cre;actB2:LoxP-STOP-LoxP-DsRedEx embryo showing a GFP+ cell undergoing EHT. (F,F′) A representative Kaede+ cell in the trunk of a gata2b:Gal4;UAS:Kaede embryo before (F) and after (F′) photoconversion. Blue dashed lines indicate the approximate location of DA and PCV. (G) Thymus of a 4dpf gata2b:Gal4;UAS:Kaede embryo that experienced photoconversion of a single aortic gata2b+ cell in the trunk at 46hpf. White dashed line highlights the thymus region. T, thymus; K, kidney; A, aorta; V, vein.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRedx hzm3Tg; s898Tg; sd32Tg; sd5Tg control Long-pec hematopoietic cell IHC
Long-pec vascular endothelium IHC
Long-pec ventral wall of dorsal aorta IHC
GFP hzm3Tg; s896Tg; sd32Tg control Prim-15 dorsal aorta endothelial cell IHC
hzm3Tg; s898Tg; sd32Tg; sd5Tg control Long-pec hematopoietic cell IHC
Long-pec ventral wall of dorsal aorta IHC
hzm3Tg; sd32Tg control Prim-5 dorsal aorta endothelial cell IFL
mu271Tg; sd32Tg control Prim-15 Not Detected branchiomotor neuron IFL
Prim-15 hematopoietic cell IFL
Protruding-mouth hematopoietic cell IFL
Protruding-mouth pronephros hematopoietic cell IFL
Protruding-mouth thymus hematopoietic cell IFL
Kaede rk8Tg; sd32Tg control Prim-15 ventral wall of dorsal aorta hematopoietic cell ISH
Day 4 thymus hematopoietic cell ISH
mCherry hzm3Tg; s896Tg; sd32Tg control Prim-15 dorsal aorta endothelial cell IHC
Prim-15 vascular endothelium IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development