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ZFIN ID: ZDB-FIG-140422-43
Holly et al., 2014 - Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development. Developmental Biology   388(2):192-204 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 5

sfrp1a/sfrp5 regulate retinal dorso-ventral patterning. To examine dorso-ventral retina patterning in sfrp1a/5 MO co-injected embryos, we employed in situ hybridization of ventral marker genes aldh1a3, ephb3a and vax2, as well as dorsal markers aldh1a2, bambia, and tbx5a at 28 and 48 hpf. At 28 hpf, sfrp1a/5 MO injected embryos display a normal domain of ventral markers compared to WT, though with slightly reduced levels (A–F). Analysis of 28 hpf dorsal retina patterning in sfrp1a/5 MO embryos also demonstrates a decreased expression of markers (G–L). By 48 hpf, in comparison to controls, sfrp1a/5 MO injected embryos display an expansion of both aldh1a3 and vax2 expression into the dorsal regions of the retina (M–P). Analysis at 48 hpf demonstrates a clear reduction of tbx5a and bambia expression domains and levels in sfrp1a/5 MO injected embryos (Q–T).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a2 WT standard conditions Prim-5 optic cup dorsal region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup dorsal region ISH
aldh1a3 WT standard conditions Prim-5 optic cup ventral region ISH
Long-pec retina ventral region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup ventral region ISH
Long-pec retina ISH
bambia WT standard conditions Prim-5 optic cup dorsal region ISH
Long-pec retina dorsal region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup dorsal region ISH
Long-pec retina dorsal region ISH
ephb3a WT standard conditions Prim-5 optic cup ventral region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup ventral region ISH
tbx5a WT standard conditions Prim-5 optic cup dorsal region ISH
Long-pec retina dorsal region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup dorsal region ISH
Long-pec retina dorsal region ISH
vax2 WT standard conditions Prim-5 optic cup ventral region ISH
Long-pec retina ventral region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup ventral region ISH
Long-pec retina ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 388(2), Holly, V.L., Widen, S.A., Famulski, J.K., and Waskiewicz, A.J., Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development, 192-204, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.