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ZFIN ID: ZDB-FIG-140422-51
Holly et al., 2014 - Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development. Developmental Biology   388(2):192-204 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5

Fig. S5

Sfrp1a/5 loss of function does not affect nasal-temporal retinal patterning. Having observed alterations in dorso-ventral patterning in sfrp1a/5 morpholino (MO) injected embryos, we sought to analyze naso-temporal pattering by performing in situ hybridization for temporal (foxd1) and nasal (foxg1a) retina markers. Compared to WT, sfrp1a/5 MO injected embryos display no change in the expression pattern of foxg1a (A-B) (n=17/17) or foxd1 (C-D) (n=14/14) at 28 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxd1 WT standard conditions Prim-5 optic cup posterior region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup posterior region ISH
foxg1a WT standard conditions Prim-5 optic cup anterior region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup anterior region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 388(2), Holly, V.L., Widen, S.A., Famulski, J.K., and Waskiewicz, A.J., Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development, 192-204, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.