ZFIN ID: ZDB-FIG-140422-42
Holly et al., 2014 - Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development. Developmental Biology   388(2):192-204 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4

sfrp1a/sfrp5 positively regulate Wnt signaling. To determine the function of Sfrp proteins in regulating Wnt signaling, we examined wnt ligand mRNA expression and a transgene reporter of Wnt signaling using whole mount in situ hybridization and quantitative PCR. In situ hybridization for eGFP mRNA in the Tg(TOP:dGFP) line shows a decrease in eGFP expression in sfrp1a/5 MO co-injected embryos, indicating a decrease in Wnt signaling at 28 hpf (A,B). The effects of Sfrp depletion on eGFP expression were confirmed using quantitative RT-PCR (C, NNN, p<0.001). In situ hybridization of Wnt ligands wnt2 and wnt8b at 28 hpf show an expansion in the expression domain of wnt2 and wnt8b in the dorsal retina in sfrp1a/5 MO injected embryos (D–G). qRT-PCR confirmed a 1.33 fold change in wnt2 and 1.38 fold change in wnt8b expression (H, NNN, p<0.005).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP w25Tg standard conditions Prim-5 optic cup ISH
w25Tg + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup ISH
wnt2 WT standard conditions Prim-5 optic cup dorsal region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup dorsal region ISH
wnt8b WT standard conditions Prim-5 optic cup dorsal region ISH
WT + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 optic cup dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w25Tg + MO2-sfrp5 + MO3-sfrp1a standard conditions Prim-5 Wnt signaling pathway decreased process quality, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 388(2), Holly, V.L., Widen, S.A., Famulski, J.K., and Waskiewicz, A.J., Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development, 192-204, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.