ZFIN ID: ZDB-FIG-110712-6
Slagle et al., 2011 - Nodal-Dependent Mesendoderm Specification Requires the Combinatorial Activities of FoxH1 and Eomesodermin. PLoS Genetics   7(5):e1002072 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to 90%-epiboly

Fig. 6

Eomes inhibition enhances the Nodal signaling and mesendoderm deficiencies of MZmid.

Markers for various populations of mesendoderm derivatives were analyzed in wild-type and MZmid embryos in which Eomes function was inhibited. Eomes inhibition in wild-type embryos has a minimal effect on expression of most markers, whereas in an MZmid background it has significant effects on mesendoderm-derived tissues, approaching MZoep levels for most markers. Arrowheads indicate Nodal-independent expression of bon in MZoep mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
drl AB standard conditions 90%-epiboly prechordal plate ISH
90%-epiboly ventral mesoderm ISH
foxh1pr1/pr1 standard conditions 90%-epiboly prechordal plate ISH
90%-epiboly ventral mesoderm ISH
tdgf1tz257/tz257 standard conditions 90%-epiboly prechordal plate ISH
90%-epiboly Not Detected ventral mesoderm ISH
mixl1 AB standard conditions 30%-epiboly margin ISH
foxh1pr1/pr1 standard conditions 30%-epiboly margin ISH
tdgf1tz257/tz257 standard conditions 30%-epiboly margin ISH
noto AB standard conditions 30%-epiboly presumptive shield ISH
foxh1pr1/pr1 standard conditions 30%-epiboly presumptive shield ISH
tdgf1tz257/tz257 standard conditions 30%-epiboly presumptive shield ISH
pax2a AB standard conditions 90%-epiboly mesoderm ISH
90%-epiboly midbrain hindbrain boundary neural plate ISH
foxh1pr1/pr1 standard conditions 90%-epiboly mesoderm ISH
90%-epiboly midbrain hindbrain boundary neural plate ISH
tdgf1tz257/tz257 standard conditions 90%-epiboly Not Detected mesoderm ISH
90%-epiboly midbrain hindbrain boundary neural plate ISH
tbx16 AB standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
foxh1pr1/pr1 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
tdgf1tz257/tz257 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
tbxta AB standard conditions 30%-epiboly margin ISH
foxh1pr1/pr1 standard conditions 30%-epiboly margin ISH
tdgf1tz257/tz257 standard conditions 30%-epiboly margin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.