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ZFIN ID: ZDB-FIG-110712-8
Slagle et al., 2011 - Nodal-Dependent Mesendoderm Specification Requires the Combinatorial Activities of FoxH1 and Eomesodermin. PLoS Genetics   7(5):e1002072 Full text @ PLoS Genet.
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. S2

Effects of FoxH1 knockdown on MZsur and MZmid mutants. MZsur and MZmid embryos were injected with 4 ng FoxH1MO to assess the effect of inhibiting production of mutant FoxH1 proteins on development. A majority of injected MZsur embryos lack notochords (115/187 without notochords); injection into MZmid mutants never rescues notochord formation (0/182 with notochords). 15/182 injected MZmid mutants had slight defects including delayed development and nonspecific necrosis which may be injection artifacts. 4/182 displayed midline bifurcations. In 5/182 embryos, injection of FoxH1MO into MZmid caused splitting of the normally fused eye field into two eyes. This effect is most likely related to the published role of the Foxh1 DNA-binding domain in inhibiting the mixl1 promoter via recruitment of Gsc protein in mouse _ [41] (see Discussion for more details).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxh1m768/m768 standard conditions Prim-5 notochord morphology, abnormal
foxh1m768/m768 + MO1-foxh1 standard conditions Prim-5 notochord aplastic, abnormal
foxh1pr1/pr1 standard conditions Prim-5 notochord aplastic, abnormal
foxh1pr1/pr1 + MO1-foxh1 standard conditions Prim-5 notochord aplastic, abnormal
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.