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ZFIN ID: ZDB-FIG-110712-2
Slagle et al., 2011 - Nodal-Dependent Mesendoderm Specification Requires the Combinatorial Activities of FoxH1 and Eomesodermin. PLoS Genetics   7(5):e1002072 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to 90%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: 30%-epiboly to 90%-epiboly

Fig. 2

Nodal-dependent tissue specification is differentially disrupted in FoxH1 mutants.

(A–L) Mesendoderm marker expression in wild-type and maternal-zygotic FoxH1 mutants at 40–50% epiboly. Dorsal is to the right; A–C, G–L are animal views, D–F are lateral views. Note the dorsal reduction of ntl expression in MZsur (E) and MZmid (F). (M–R) Axial mesoderm and endoderm marker expression at 90% epiboly, viewed dorsally with anterior up.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gsc AB standard conditions 30%-epiboly presumptive shield ISH
90%-epiboly prechordal plate ISH
foxh1m768/m768 standard conditions 30%-epiboly presumptive shield ISH
90%-epiboly prechordal plate ISH
foxh1pr1/pr1 standard conditions 30%-epiboly presumptive shield ISH
90%-epiboly prechordal plate ISH
mixl1 AB standard conditions 30%-epiboly margin ISH
foxh1m768/m768 standard conditions 30%-epiboly margin ISH
foxh1pr1/pr1 standard conditions 30%-epiboly margin ISH
sox32 AB standard conditions 90%-epiboly endoderm ISH
foxh1m768/m768 standard conditions 90%-epiboly endoderm ISH
foxh1pr1/pr1 standard conditions 90%-epiboly endoderm ISH
tbxta AB standard conditions 30%-epiboly margin ISH
90%-epiboly axial chorda mesoderm ISH
foxh1m768/m768 standard conditions 30%-epiboly margin ISH
90%-epiboly axial chorda mesoderm ISH
foxh1pr1/pr1 standard conditions 30%-epiboly margin ISH
90%-epiboly Not Detected axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxh1m768/m768 standard conditions 30%-epiboly - 90%-epiboly axial chorda mesoderm morphology, abnormal
30%-epiboly - 90%-epiboly axial mesodermal cell fate specification disrupted, abnormal
foxh1pr1/pr1 standard conditions 30%-epiboly - 90%-epiboly axial chorda mesoderm aplastic, abnormal
30%-epiboly - 90%-epiboly axial mesodermal cell fate specification disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.