ZFIN Gene: tbx16

ZFIN ID: ZDB-GENE-990615-5

ZFIN ID: ZDB-GENE-990615-5

Gene Name:

T-box transcription factor 16

Gene Symbol:

tbx16 Nomenclature History

Previous Names:

cb103 (1), id:ibd2185 (1), spadetail, spt-1 (1), spt (1), wu:fa17g07 (1), ZF-SPT (1), zgc:109817

Gene Type:

protein_coding_gene

Location:

Chr: 8 Mapping Details/Browsers

Description:

Exhibits transcription regulatory region sequence-specific DNA binding activity. Involved in several processes, including animal organ development; blood vessel morphogenesis; and regulation of embryonic development. Localizes to nuclear chromatin. Is expressed in several structures, including forerunner cell group; germ ring; mesoderm; presumptive mesoderm; and spinal cord.

Genome Resources:

Alliance (1), Gene:30264 (1), Ensembl(GRCz11):ENSDARG00000007329 (1)

GENE EXPRESSION
All Expression Data:	88 figures from 61 publications
Directly Submitted Expression Data:	6 figures (10 images) from Thisse et al., 2001 [cb103]
	4 figures (10 images) from Kudoh et al., 2001 [ibd2185]
Wild-type Stages, Structures:	Cleavage:64-cell (2.0h-2.25h) to Pharyngula:Prim-25 (36.0h-42.0h)
	adaxial cell nucleus adaxial cell anterior axial hypoblast DoLA epiblast forerunner cell group germ ring lateral region germ ring nucleus germ ring ventral region germ ring hypoblast margin ventro-lateral region margin mesoderm neural tube neuron paraxial mesoderm polster prechordal plate presumptive mesoderm presumptive paraxial mesoderm presumptive segmental plate segmental plate nucleus segmental plate somite spinal cord neuron spinal cord tail bud epiblast tail bud nucleus tail bud unspecified whole organism cytoplasm whole organism nucleus whole organism
High Throughput Expression:	GEO (1) Expression Atlas (1)

MUTATIONS AND SEQUENCE TARGETING REAGENTS

Allele	Type	Localization	Consequence	Mutagen	Suppliers
b104	Indel	Unknown	Premature Stop	SPONTANEOUS	Zebrafish International Resource Center (ZIRC) (order this)
hi2109Tg	Transgenic Insertion	Intron 1	Unknown	DNA
hi3714Tg	Transgenic Insertion	Intron 1	Unknown	DNA	Zebrafish International Resource Center (ZIRC) (order this)
kt378b	Point Mutation	Acceptor Splice Site of Intron 1	Unknown	ENU
la016429Tg	Transgenic Insertion	Unknown	Unknown	DNA	Zebrafish International Resource Center (ZIRC) (order this)
la026643Tg	Transgenic Insertion	Unknown	Unknown	DNA	Zebrafish International Resource Center (ZIRC) (order this)
la026644Tg	Transgenic Insertion	Unknown	Unknown	DNA	Zebrafish International Resource Center (ZIRC) (order this)
la029508Tg	Transgenic Insertion	Unknown	Unknown	DNA
m423	Point Mutation	Unknown	Unknown	ENU	Zebrafish International Resource Center (ZIRC) (order this)
tbx16_unspecified	Unspecified	Unknown	Unknown
thp003	Point Mutation	Unknown	Missense	ENU
tm41	Unknown	Unknown	Unknown	ENU	European Zebrafish Resource Center (EZRC) (order this)
tq5	Unknown	Unknown	Unknown	ENU	European Zebrafish Resource Center (EZRC) (order this)

Sequence Targeting Reagents

Targeting Reagent	Created Alleles	Publications
CRISPR1-tbx16		3
CRISPR2-tbx16		2
CRISPR3-tbx16		1
CRISPR4-tbx16		1
CRISPR5-tbx16		1
CRISPR6-tbx16		1
MO1-tbx16	N/A	11
MO2-tbx16	N/A	11
MO3-tbx16	N/A	11
MO4-tbx16	N/A	2
MO5-tbx16	N/A	1
MO6-tbx16	N/A	2

PHENOTYPE

Data:

59 figures from 32 publications

Observed in:

DISEASE ASSOCIATED WITH tbx16 HUMAN ORTHOLOG No data available

DISEASE ASSOCIATED WITH tbx16 VIA EXPERIMENTAL MODELS No data available

GENE ONTOLOGY

Ontology	GO Term
Biological Process	digestive tract development (more)
Cellular Component	nuclear chromatin (more)
Molecular Function	DNA binding (more)

GO Terms (all 41)

PROTEIN FAMILIES, DOMAINS AND SITES No links to external sites available

TRANSCRIPTS
Type	Name	Length (nt)	Analysis
mRNA	tbx16-201 (1)	4949	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT

Browsers: UCSC, NCBI, Ensembl, ZFIN

INTERACTIONS AND PATHWAYS

ANTIBODIES

Name	Type	Isotype	Host Organism	Assay	Source	Publications
AB3-tbx16	monoclonal	IgG2a	Mouse			1
Ab2-tbx16	polyclonal		Mouse	IHC		3

PLASMIDS No data available

CONSTRUCTS WITH SEQUENCES FROM tbx16

Construct	Regulatory Regions	Coding Sequences	Species	Tg Lines	Publications
Tg(-1.2tbx16:TagRFP)	tbx16	TagRFP	Danio rerio	1	1
Tg(-1.2tbx16:TagRFP,cryaa:GFP)	cryaa, tbx16	GFP, TagRFP	Danio rerio	1	1
Tg(HSE:tbx16-MYC,EGFP)		EGFP, tbx16	Danio rerio	1	2
Tg(hsp70l:tbx16-2A-NLS-KikGR)	hsp70l	2A, KikGR, NLS, tbx16	Danio rerio	1	1
Tg(hsp70l:tbx16-ENR-2A-NLS-KikGR)	hsp70l	2A, ENR, KikGR, NLS, tbx16	Danio rerio	1	1
Tg(tbx16:EGFP)	tbx16	EGFP	Danio rerio	4	5
Tg(tbx16:mRFP)	tbx16	mRFP	Danio rerio	1	1
Tg2(hsp70l:tbx16-2A-NLS-KikGR)	hsp70l	2A, KikGR, NLS, tbx16	Danio rerio	1	1

MARKER RELATIONSHIPS

tbx16 Contained in:	[BAC] CH73-313L22 (1)
tbx16 Encodes:	[EST] cb103 (1) (order this), fa17g07 (1), ibd2185 [STS] chunp30288 [cDNA] MGC:109817 (1), MGC:193075 (1)

SEQUENCE INFORMATION

Type	Accession #	Length (nt/aa)	Analysis
RNA	RefSeq:NM_131058 (1)	2561 nt	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Genomic	GenBank:CU459194 (1)	101559 nt	Select Tool MegaBLAST Ensembl
Polypeptide	UniProtKB:B8JL25 (1)	470 aa	Select Tool ZFIN BLAST SIB BLAST Ensembl Protein Blast

Sequence Information (all 23)

ORTHOLOGY for tbx16 ( Chr: 8 )

CITATIONS (255)

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