header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-110712-3
Slagle et al., 2011 - Nodal-Dependent Mesendoderm Specification Requires the Combinatorial Activities of FoxH1 and Eomesodermin. PLoS Genetics   7(5):e1002072 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 30%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 3

sur retains more Nodal transduction capability than mid.

(A) Genetic interactions between the sur and mid alleles, demonstrating the ability of sur to partially rescue the loss of notochord caused by mid. Lower panels are enlargements showing the structures of the notochords at 24 hpf; red brackets indicate notochord domains. (B) Nodal overexpression in maternal-zygotic FoxH1 mutants. 50 pg sqt RNA was injected into wild-type, MZsur, and MZmid embryos at the one-cell stage, and embryos were assayed for Nodal target gene expression at 30–40% epiboly. Note the greater ability of MZsur embryos to respond to ectopic sqt compared to the MZmid response. (C) The mid mutation perturbs activin-like signaling. MZoep and MZmid embryos were injected with RNA encoding either a Xenopus activin homologue (2.5 pg) or an activated form of mouse Smad2 (100 pg). Responses were assayed by observing ntl expression at 30–40% epiboly.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gsc AB standard conditions 30%-epiboly presumptive shield ISH
foxh1m768/m768 standard conditions 30%-epiboly presumptive shield ISH
foxh1pr1/pr1 standard conditions 30%-epiboly presumptive shield ISH
tbxta AB standard conditions 30%-epiboly margin ISH
foxh1m768/m768 standard conditions 30%-epiboly margin ISH
foxh1pr1/pr1 standard conditions 30%-epiboly margin ISH
tdgf1tz257/tz257 standard conditions 30%-epiboly margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxh1m768/+; foxh1pr1/+ standard conditions Prim-5 notochord morphology, abnormal
foxh1m768/m768 standard conditions Prim-5 notochord morphology, abnormal
foxh1pr1/pr1 standard conditions Prim-5 notochord aplastic, abnormal
Prim-5 somite fused with somite, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.