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ZIRC
ZFIN ID: ZDB-FIG-110712-5
Slagle et al., 2011 - Nodal-Dependent Mesendoderm Specification Requires the Combinatorial Activities of FoxH1 and Eomesodermin. PLoS Genetics   7(5):e1002072 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 5

Inhibiting Nodal signals in MZmid mutants.

Wild-type (A), uninjected MZmid (B), and injected MZmid (C–E) embryos compared to a complete loss of Nodal signaling in MZoep (F) mutants. Inhibiting bon function (3 ng bonMO) in an MZmid background further impairs anterior development but does not affect tail development (C). Blocking eomes function (15–20 pg eomes-enR mRNA; D) resembles both a loss of the Nodal ligands cyc and sqt (8 ng each cycMO+sqtMO; E) and MZoep (F), indicating that Nodal signaling is occurring in MZmid mutants and is mediated by Eomes.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxh1pr1/pr1 standard conditions Prim-5 brain morphology, abnormal
Prim-5 extension decreased length, abnormal
Prim-5 extension increased thickness, abnormal
Prim-5 neural plate anterior/posterior regionalization disrupted, abnormal
Prim-5 notochord aplastic, abnormal
Prim-5 pericardium edematous, abnormal
Prim-5 somite fused with somite, abnormal
Prim-5 whole organism anterior-posterior axis curved ventral, abnormal
foxh1pr1/pr1 + MO1-mixl1 standard conditions Prim-5 brain morphology, abnormal
Prim-5 extension decreased length, abnormal
Prim-5 extension increased thickness, abnormal
Prim-5 head hypoplastic, abnormal
Prim-5 neural plate anterior/posterior regionalization arrested, abnormal
Prim-5 notochord aplastic, abnormal
Prim-5 pericardium edematous, abnormal
Prim-5 somite fused with somite, abnormal
Prim-5 whole organism anterior-posterior axis curved ventral, abnormal
foxh1pr1/pr1 + MO1-ndr2 + MO4-ndr1 standard conditions Prim-5 dorsal/ventral pattern formation disrupted, abnormal
Prim-5 extension aplastic, abnormal
Prim-5 eye malformed, abnormal
Prim-5 head hypoplastic, abnormal
Prim-5 head morphology, abnormal
Prim-5 post-vent region malformed, abnormal
Prim-5 whole organism anterior-posterior axis curved ventral, abnormal
tdgf1tz257/tz257 standard conditions Prim-5 dorsal/ventral pattern formation disrupted, abnormal
Prim-5 extension aplastic, abnormal
Prim-5 eye malformed, abnormal
Prim-5 head hypoplastic, abnormal
Prim-5 head morphology, abnormal
Prim-5 post-vent region malformed, abnormal
Prim-5 whole organism anterior-posterior axis curved ventral, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.