ZFIN ID: ZDB-FIG-050314-9
Secretory cells express the Notch ligand Delta and their numbers are increased in aeiAR33, a mutant lacking DeltaD. (A) Intestine of adult mouse heterozygous for a lacZ knock-in at the Delta1 (Dll1) locus. ß-Galactosidase activity (blue) is a reporter for present or past expression of Delta1 expression; the stain is seen in scattered cells, many of which can be clearly identified as goblet cells. (B) Expression of Notch pathway components in zebrafish gut, analysed by RT-PCR. deltaC and deltaD are strongly expressed in the gut, while deltaA and deltaB are not. (C) DeltaD (zdd-2 immunostain in green, arrows) is visible in a subset of secretory cells (2F11, red) in the wild-type zebrafish intestine at 4 days. (D,E) Sections of intestinal bulb of 5-day-old wild-type larvae (D) and aeiAR33 homozygotes (E) stained with 2F11; TOPRO-3 nuclear stain is red. (F) The proportion of 2F11-positive cells is increased all along the length of the gut in aeiAR33 when compared with wild type; data points show mean and s.e.m. from counts of sections of 12 larvae of each genotype. (G) Total numbers of epithelial cells per section for the same set of specimens.
Antibody Labeling Details No data available
Phenotype Details No data available
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