ZFIN ID: ZDB-FIG-050314-9
Crosnier et al., 2005 - Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine. Development (Cambridge, England)   132(5):1093-1104 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Day 4 to Days 45-89

Fig. 4 Secretory cells express the Notch ligand Delta and their numbers are increased in aeiAR33, a mutant lacking DeltaD. (A) Intestine of adult mouse heterozygous for a lacZ knock-in at the Delta1 (Dll1) locus. ß-Galactosidase activity (blue) is a reporter for present or past expression of Delta1 expression; the stain is seen in scattered cells, many of which can be clearly identified as goblet cells. (B) Expression of Notch pathway components in zebrafish gut, analysed by RT-PCR. deltaC and deltaD are strongly expressed in the gut, while deltaA and deltaB are not. (C) DeltaD (zdd-2 immunostain in green, arrows) is visible in a subset of secretory cells (2F11, red) in the wild-type zebrafish intestine at 4 days. (D,E) Sections of intestinal bulb of 5-day-old wild-type larvae (D) and aeiAR33 homozygotes (E) stained with 2F11; TOPRO-3 nuclear stain is red. (F) The proportion of 2F11-positive cells is increased all along the length of the gut in aeiAR33 when compared with wild type; data points show mean and s.e.m. from counts of sections of 12 larvae of each genotype. (G) Total numbers of epithelial cells per section for the same set of specimens.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dla WT standard conditions Days 30-44 to Days 45-89 Not Detected gut RTPCR
dlb WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
dlc WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
dld WT standard conditions Day 4 gut IHC
Days 30-44 to Days 45-89 gut RTPCR
jag1a WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
jag1b WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
jag2b WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
notch1a WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
notch1b WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
notch2 WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
notch3 WT standard conditions Days 30-44 to Days 45-89 gut RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development