PUBLICATION

Zebrafish Neural Tube Morphogenesis Requires Scribble-Dependent Oriented Cell Divisions

Authors

Zigman, M., Trinh, L.A., Fraser, S.E., and Moens, C.B.

ID

ZDB-PUB-110103-23

Date

2011

Source

Current biology : CB 21(1): 79-86 (Journal)

Registered Authors

Fraser, Scott E., Moens, Cecilia, Trinh, Le, Zigman, Mihaela

Keywords

none

MeSH Terms

Mitosis/physiology*
Membrane Proteins/genetics
Membrane Proteins/metabolism*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Animals
Gene Expression Regulation, Developmental/physiology*
Zebrafish/embryology*
Zebrafish/metabolism
Neural Tube/cytology
Neural Tube/embryology*

(all 11)

PubMed

21185191 Full text @ Curr. Biol.

Abstract

How control of subcellular events in single cells determines morphogenesis on the scale of the tissue is largely unresolved. The stereotyped cross-midline mitoses of progenitors in the zebrafish neural keel [1-4] provide a unique experimental paradigm for defining the role and control of single-cell orientation for tissue-level morphogenesis in vivo. We show here that the coordinated orientation of individual progenitor cell division in the neural keel is the cellular determinant required for morphogenesis into a neural tube epithelium with a single straight lumen. We find that Scribble is required for oriented cell division and that its function in this process is independent of canonical apicobasal and planar polarity pathways. We identify a role for Scribble in controlling clustering of α-catenin foci in dividing progenitors. Loss of either Scrib or N-cadherin results in abnormally oriented mitoses, reduced cross-midline cell divisions, and similar neural tube defects. We propose that Scribble-dependent nascent cell-cell adhesion clusters between neuroepithelial progenitors contribute to define orientation of their cell division. Finally, our data demonstrate that while oriented mitoses of individual cells determine neural tube architecture, the tissue can in turn feed back on its constituent cells to define their polarization and cell division orientation to ensure robust tissue morphogenesis.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
Citrine	ctnna1^ct3aGt	control	5-9 somites	neural keel neuroepithelial cell	IFL	Fig. 3
Citrine	ctnna1^ct3aGt	control	5-9 somites	neuroepithelial cell catenin complex	IFL	Fig. 3
Citrine	ctnna1^ct3aGt	control	5-9 somites	neuroepithelial cell cell cortex	IFL	Fig. 3
Citrine	ctnna1^ct3aGt; kca66Tg	standard conditions	5-9 somites	neural keel neuroepithelial cell	IFL	Fig. 3
Citrine	ctnna1^ct3aGt; kca66Tg	standard conditions	5-9 somites	neuroepithelial cell catenin complex	IFL	Fig. 3
Citrine	ctnna1^ct3aGt; kca66Tg	standard conditions	5-9 somites	neuroepithelial cell cell cortex	IFL	Fig. 3
Citrine	ctnna1^ct3aGt + MO1-cdh2	standard conditions	5-9 somites	neural keel neuroepithelial cell	IFL	Fig. 3
Citrine	ctnna1^ct3aGt + MO1-cdh2	standard conditions	5-9 somites	neuroepithelial cell catenin complex	IFL	Fig. 3
Citrine	ctnna1^ct3aGt + MO1-cdh2	standard conditions	5-9 somites	neuroepithelial cell cell cortex	IFL	Fig. 3
Citrine	ctnna1^ct3aGt + MO1-scrib	standard conditions	5-9 somites	neural keel neuroepithelial cell	IFL	Fig. 3

1 - 10 of 15

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-dvl2	standard conditions	5-9 somites	establishment of mitotic spindle orientation process quality, normal	Fig. 2
WT + MO1-dvl2	standard conditions	5-9 somites	neuroepithelial cell mitotic spindle direction, normal	Fig. 2
WT + MO1-prickle1a + MO1-prickle1b	standard conditions	5-9 somites	establishment of mitotic spindle orientation process quality, normal	Fig. 2
WT + MO1-prickle1a + MO1-prickle1b	standard conditions	5-9 somites	neuroepithelial cell mitotic spindle direction, normal	Fig. 2
WT + MO1-scrib	standard conditions	5-9 somites	establishment of mitotic spindle orientation decreased process quality, abnormal	Fig. 1
WT + MO1-scrib	standard conditions	5-9 somites	neural keel neuroepithelial cell disorganized, abnormal	Fig. 1
WT + MO1-scrib	standard conditions	5-9 somites	neuroepithelial cell mitotic spindle direction, abnormal	Fig. 1
WT + MO1-scrib	standard conditions	26+ somites	neural tube neuroepithelial cell disorganized, abnormal	Fig. 1
WT + MO1-scrib	standard conditions	26+ somites	secondary neural tube formation decreased process quality, abnormal	Fig. 1
cdh2^m117/m117	standard conditions	5-9 somites	fourth ventricle malformed, abnormal	Fig. 3

1 - 10 of 34

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ct3aGt	Gt(FRT-LOXP-Citrine-LOX2272-mCherry-LOXP-LOX2272-FRT)	Transgenic Insertion	ctnna1
fh266		Point Mutation	pard6gb
kca66Tg	Tg(h2az2a:h2az2a-GFP)	Transgenic Insertion
m117		Point Mutation	cdh2
m209		Point Mutation	vangl2
rw16		Point Mutation	scrib

1 - 6 of 6

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
cdh2	MO1-cdh2	MRPHLNO
dvl2	MO1-dvl2	MRPHLNO
prickle1a	MO1-prickle1a	MRPHLNO
prickle1b	MO1-prickle1b	MRPHLNO
prickle1b	MO2-prickle1b	MRPHLNO
scrib	MO1-scrib	MRPHLNO
vangl2	MO1-vangl2	MRPHLNO

1 - 7 of 7

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Fish

Fish
cdh2^m117/m117
ctnna1^ct3aGt
ctnna1^ct3aGt; kca66Tg
ctnna1^ct3aGt + MO1-cdh2
ctnna1^ct3aGt + MO1-scrib
kca66Tg
pard6gb^fh266/fh266
pard6gb^fh266/fh266; scrib^rw16/rw16
scrib^rw16/rw16
vangl2^m209/m209

1 - 10 of 14

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
Citrine	EFG	Citrine
GFP	EFG	GFP

Mapping

Zigman et al., 2011 ZDB-PUB-110103-23 PMID:21185191

Zebrafish Neural Tube Morphogenesis Requires Scribble-Dependent Oriented Cell Divisions

Zigman et al., 2011

ZDB-PUB-110103-23
PMID:21185191