FIGURE

Fig. 2

ID

ZDB-FIG-251110-2

Publication

Yu et al., 2025 - LPS disrupts erythroid-myeloid balance in zebrafish via Jak2/Stat3-Hif1a signaling

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Fig. 2

Acute LPS treatment leads to a bias in erythroid-myeloid hematopoietic differentiation. (A) Schematic of the zebrafish experimental design, detailing procedures, groupings, and imaging techniques. (B) Relative mRNA levels of inflammatory markers. (C) O-dianisidine staining for erythroid cells in the caudal hematopoietic tissue (CHT) and their quantification. (D) Fluorescence in CHT of Tg(gata1a:DsRed) transgenic zebrafish and their quantification. (E) Sudan Black staining for neutrophils in the yolk sac and their quantification. (F) Fluorescence in the yolk sac of Tg(lyz:EGFP) transgenic zebrafish and their quantification. (G) Neutral red staining for macrophages in larvae heads and their quantification. (H) Fluorescence in the yolk sac of Tg(mpeg1:EGFP) transgenic zebrafish and their quantification. (I) mRNA expression analysis of erythroid and myeloid differentiation markers. In C-H, n = replicates per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Expression Data

Genes:	csf3a DsRed EGFP gata1a hbae3 il1b il6 mpx nos2b tnfb
Fish:	ihb20Tg/ihb20Tg nz117Tg/nz117Tg sd2Tg/sd2Tg AB
Condition:	chemical treatment by injection: lipopolysaccharide
Anatomical Terms:	caudal hematopoietic tissue macrophage caudal hematopoietic tissue neutrophil caudal hematopoietic tissue nucleate erythrocyte whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ihb20Tg/ihb20Tg nz117Tg/nz117Tg sd2Tg/sd2Tg AB
Condition:	chemical treatment by injection: lipopolysaccharide
Observed In:	caudal hematopoietic tissue macrophage caudal hematopoietic tissue neutrophil caudal hematopoietic tissue nucleate erythrocyte whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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