FIGURE

Figure 3

ID

ZDB-FIG-210307-98

Publication

Li et al., 2021 - Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing

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Figure 3

Increased apoptosis and activated p53 pathway in RPCs of prpf31⁻^/⁻ zebrafish. (A) TUNEL staining showed numerous apoptotic cells in the retina, brain and posterior segment of spinal cord in prpf31 mutants at 48 hpf. Injection of wild-type prpf31 mRNAs reduced the apoptotic cells to a normal level. Dotted lines indicate the boundary of two images from the same embryo. White arrows, apoptotic signals. Scale bar, 100 μm. (B) The up-regulation of p53 pathway genes in prpf31 mutants at 36 hpf as detected by qPCR. (C) Deletion of p53 in prpf31 mutants significantly reduced cell apoptosis, but could not rescue the differentiation defects of RPCs. Scale bar, 100 μm. (D−F) The quantitative analysis of TUNEL positive cells, Sox2 positive cells and Islet1 positive cells shown in (C). n = 9 for each panel. Scale bar, 100 μm.

Expression Data

Genes:	baxa bbc3 bcl2a casp8 ccng1 cdkn1a gadd45ba mdm2 tp53
Antibodies:	Ab6-isl1 Ab11-sox2
Fish:	WT prpf31^hzu12/hzu12 prpf31^hzu12/hzu12; tp53^{ihb135/ihb135}
Anatomical Terms:	retina whole organism
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prpf31^hzu12/hzu12 prpf31^hzu12/hzu12; tp53^{ihb135/ihb135}
Observed In:	brain apoptotic process multi fate stem cell neural retina development retina retina apoptotic process spinal cord apoptotic process whole organism
Stage Range:	Prim-25 to Long-pec

Phenotype Detail

Acknowledgments

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