FIGURE

Figure 2

ID

ZDB-FIG-210307-97

Publication

Li et al., 2021 - Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing

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Figure 2

Deletion of Prpf31 impaired RPCs differentiation. (A) Retinal sections of WT, prpf31⁻^/⁻ and prpf31⁻^/⁻ mRNA-rescued embryos were immunostained with Sox2 (marker for RPCs), Islet1 (marker for neuron cells), Zpr1 (marker for cone cells), and Zpr3 (marker for rod cells) antibodies at 48 or 60 hpf. n = 8 for each panel. V, ventral side, D, dorsal side. Scale bar, 100 μm. (B) The distributions of Neurod1:EGFP (specialized neurons, upper panels) and Huc:EGFP (post-mitotic neurons, lower panels) labeled cells in whole-mount retinas from WT and prpf31⁻^/− transgene zebrafish. The dashed circles shown the eyes and lens respectively. n ≥ 7 for each panel; Scale bar, 100 μm. (C) In situ staining of markers for RPCs (ccnd1, vsx2) and for neural precursors (atoh7, crx1), specialized neurons (neurod1) and mature neurons (tuba1) at 48 and 60 hpf. The accumulation of RPCs and reductions of differentiated neurons are shown. Scale bar, 100 μm.

Expression Data

Genes:	atoh7 ccnd1 crx EGFP neurod1 vsx2
Antibodies:	Ab6-isl1 Ab11-sox2 zpr-1 zpr-3
Fish:	WT prpf31^hzu12/hzu12 knu3Tg nl1Tg prpf31^hzu12/hzu12; knu3Tg prpf31^hzu12/hzu12; nl1Tg
Anatomical Terms:	eye photoreceptor cell retina retina multi fate stem cell retina neuron retinal cone cell retinal inner nuclear layer retinal rod cell
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prpf31^hzu12/hzu12 prpf31^hzu12/hzu12; knu3Tg prpf31^hzu12/hzu12; nl1Tg
Observed In:	eye photoreceptor cell multi fate stem cell neural retina development neuron retina retina multi fate stem cell retina neuron retinal inner nuclear layer
Stage Range:	Long-pec to Pec-fin

Phenotype Detail

Acknowledgments

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