FIGURE

Figure 6

ID

ZDB-FIG-210307-101

Publication

Li et al., 2021 - Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing

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Figure 6

The defective retinal development in prpf31 mutants can be rescued by injection of wild-type but not RP mutant PRPF31 mRNA. (A,B) The retina phenotype of the siblings and mutants injected with human PRPF31 wild-type mRNA or RP mutant mRNA. BF, bright field; Islet1, neurons marker; pH3, M-phase marker; TUNEL, apoptosis marker; phalloidin, labeled the lamination of the retina. (C−F) The statistical data show the numbers of specific positive cells in (A), (B). Note that wild type mRNA can significantly rescued the deficient phenotypes in mutant retina, while p.Lys257fs*277 mutant mRNA has almost no effect, the p.Arg372Glnfs*99 mutation seems to have a negative effect on siblings and mutants at 60 hpf. The number of embryos used in each assay was 6. Scale bar, 100μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.