ZFIN ID: ZDB-FIG-191230-1670
Fillatre et al., 2019 - TEADs, Yap, Taz, Vgll4s transcription factors control the establishment of Left-Right asymmetry in Zebrafish. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Shield to 90%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to 90%-epiboly

Figure 4 Whole-mount in situ hybridization for genes that are expressed in DFCs at 70–90% epiboly in control (Ctrl) embryos and that are strongly downregulated in Vgll4l MO knockdown. Embryos are in dorsal view, animal pole to the top (A–L), in vegetal pole view dorsal to the right (M–W) and in lateral view anterior to the top dorsal to the right (X). Name of the genes probed is indicated in between control (top) and Vgll4l loss of function embryos (bottom).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
abcc6a AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
atp6ap1b AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
cdc14aa AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
cfap45 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly Not Detected forerunner cell group ISH
cftr AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly Not Detected forerunner cell group ISH
cldn5a AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly Not Detected forerunner cell group ISH
daw1 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
dhrs13a.1 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly Not Detected forerunner cell group ISH
dnaaf4 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly Not Detected forerunner cell group ISH
dnmt3bb.1 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
dnmt3bb.2 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
mbd3b AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
ndr1 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly Not Detected forerunner cell group ISH
nme5 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
odad4 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
quo AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly Not Detected forerunner cell group ISH
rasgef1ba AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
rassf7b AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
si:ch73-364h19.1 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly Not Detected forerunner cell group ISH
slc35d1a AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
sypl2b AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
tekt1 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
tnfrsf21 AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
vgll4l AB/TU standard conditions Shield to 90%-epiboly forerunner cell group ISH
AB/TU + MO3-vgll4l standard conditions Shield to 90%-epiboly forerunner cell group ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU + MO3-vgll4l standard conditions Shield - 90%-epiboly forerunner cell group abcc6a expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group atp6ap1b expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group cdc14aa expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group cfap45 expression absent, abnormal
Shield - 90%-epiboly forerunner cell group cftr expression absent, abnormal
Shield - 90%-epiboly forerunner cell group cldn5a expression absent, abnormal
Shield - 90%-epiboly forerunner cell group daw1 expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group dhrs13a.1 expression absent, abnormal
Shield - 90%-epiboly forerunner cell group dnaaf4 expression absent, abnormal
Shield - 90%-epiboly forerunner cell group dnmt3bb.1 expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group dnmt3bb.2 expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group mbd3b expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group ndr1 expression absent, abnormal
Shield - 90%-epiboly forerunner cell group nme5 expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group odad4 expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group quo expression absent, abnormal
Shield - 90%-epiboly forerunner cell group rasgef1ba expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group rassf7b expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group si:ch73-364h19.1 expression absent, abnormal
Shield - 90%-epiboly forerunner cell group slc35d1a expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group sypl2b expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group tekt1 expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group tnfrsf21 expression decreased amount, abnormal
Shield - 90%-epiboly forerunner cell group vgll4l expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife