Gene
daw1
- ID
- ZDB-GENE-050419-255
- Name
- dynein assembly factor with WDR repeat domains 1
- Symbol
- daw1 Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 18 Mapping Details/Browsers
- Description
- Predicted to enable ubiquitin-like ligase-substrate adaptor activity. Involved in axonemal dynein complex assembly; cilium-dependent cell motility; and determination of left/right symmetry. Acts upstream of or within determination of heart left/right asymmetry; otolith morphogenesis; and regulation of cilium movement. Predicted to be located in ciliary basal body. Predicted to be part of SCF ubiquitin ligase complex. Is expressed in several structures, including Kupffer's vesicle; forerunner cell group; nervous system; neural tube; and solid lens vesicle. Human ortholog(s) of this gene implicated in primary ciliary dyskinesia. Orthologous to human DAW1 (dynein assembly factor with WD repeats 1).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 8 figures from 3 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- IMAGE:6904039 (41 images)
Wild Type Expression Summary
Phenotype Summary
Mutations
| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-daw1 | Bearce et al., 2022 | |
| CRISPR2-daw1 | Bearce et al., 2022 | |
| CRISPR3-daw1 | Bearce et al., 2022 | |
| CRISPR4-daw1 | Bearce et al., 2022 | |
| CRISPR5-daw1 | (2) | |
| MO1-daw1 | N/A | Gao et al., 2010 |
| MO2-daw1 | N/A | Gao et al., 2010 |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| Ciliary dyskinesia, primary, 52 | 620570 |
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Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Conserved_site | IPR019775 | WD40 repeat, conserved site |
| Family | IPR053299 | ASTRA complex-associated WD repeat |
| Homologous_superfamily | IPR015943 | WD40/YVTN repeat-like-containing domain superfamily |
| Homologous_superfamily | IPR036322 | WD40-repeat-containing domain superfamily |
| Repeat | IPR001680 | WD40 repeat |
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Domain Details Per Protein
| Protein | Additional Resources | Length | ASTRA complex-associated WD repeat | G-protein beta WD-40 repeat | WD40 repeat | WD40 repeat, conserved site | WD40-repeat-containing domain superfamily | WD40/YVTN repeat-like-containing domain superfamily |
|---|---|---|---|---|---|---|---|---|
| UniProtKB:R4GDM4 | InterPro | 418 | ||||||
| UniProtKB:F1RB29 | InterPro | 415 | ||||||
| UniProtKB:Q1LV15 | InterPro | 415 |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | DKEY-223N17 | ZFIN Curated Data | |
| Encodes | EST | IMAGE:6904039 | Thisse et al., 2004 | |
| Encodes | cDNA | MGC:153626 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:153780 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:191692 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001044352 (1) | 1734 nt | ||
| Genomic | GenBank:BX908751 (1) | 157961 nt | ||
| Polypeptide | UniProtKB:R4GDM4 (1) | 418 aa |
- Bearce, E.A., Irons, Z.H., Craig, S.B., Kuhns, C.J., Sabazali, C., Farnsworth, D.R., Miller, A.C., Grimes, D.T. (2022) Daw1 regulates the timely onset of cilia motility during development. Development (Cambridge, England). 149(12)
- Leslie, J.S., Hjeij, R., Vivante, A., Bearce, E.A., Dyer, L., Wang, J., Rawlins, L., Kennedy, J., Ubeyratna, N., Fasham, J., Irons, Z.H., Craig, S.B., Koenig, J., George, S., Pode-Shakked, B., Bolkier, Y., Barel, O., Mane, S., Frederiksen, K.K., Wenger, O., Scott, E., Cross, H.E., Lorentzen, E., Norris, D.P., Anikster, Y., Omran, H., Grimes, D.T., Crosby, A.H., Baple, E.L. (2022) Biallelic DAW1 variants cause a motile ciliopathy characterized by laterality defects and subtle ciliary beating abnormalities. Genetics in medicine : official journal of the American College of Medical Genetics. 24(11):2249-2261
- Fillatre, J., Fauny, J.D., Fels, J.A., Li, C., Goll, M., Thisse, C., Thisse, B. (2019) TEADs, Yap, Taz, Vgll4s transcription factors control the establishment of Left-Right asymmetry in Zebrafish. eLIFE. 8:
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Hsieh, F.Y., Ma, T.L., Shih, H.Y., Lin, S.J., Huang, C.W., Wang, H.Y., and Cheng, Y.C. (2013) Dner inhibits neural progenitor proliferation and induces neuronal and glial differentiation in zebrafish. Developmental Biology. 375(1):1-12
- Gao, C., Wang, G., Amack, J.D., and Mitchell, D.R. (2010) Oda16/Wdr69 is essential for axonemal dynein assembly and ciliary motility during zebrafish embryogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(8):2190-2197
- Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J., and Marra,M.A. (2002) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America. 99(26):16899-903
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