ZFIN ID: ZDB-FIG-190611-32
Turner et al., 2019 - Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits. PLoS One   14:e0211073 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 6

RNAseq analysis reveals that loss of Slbp function induces large-scale changes in gene expression levels consistent with many cells failing to transition to differentiation.

(A) Volcano plot displaying differential expressed genes between wildtype and slbpty77eembryos The red dots on the right represent the significant up regulated expressed transcripts (p < 0.01, false discovery rate (FDR) q < 0.01); the red dots on the left represent the transcripts with expression significantly down regulated (p < 0.01, FDR q < 0.01). Non-significant genes (q > 0.01) are represented by a black dot. (B) GO term categories for enriched genes. (B’, B”) Pie charts showing percentages of GO terms relating to each category for up (B) and down (B’)-regulated genes in slbpty77e mutants compared to wildtype. C) Graph showing real time PCR quantification of expression changes for genes selected from the RNAseq dataset. Samples were normalized to β-actin and wildtype values for each gene were set to 1. Fold changes in mutants were plotted relative to this value. (D-F’) Lateral views of 3dpf wildtype (D-F) and slbpty77emutant (D’-F’) heads/eyes showing expression of h2afx (D, D’); gnb3a (E,E’) and pcdh10(F,F’). Note that expression changes for h2afx and gnb3a (C) are consistent with qPCR data (yellow box in C). Scale bars: 100μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a3 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
ccnd1 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
chd7 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
col10a1a slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
crabp1b slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
elavl2 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
gnb3a WT standard conditions Protruding-mouth eye ISH
slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
Protruding-mouth eye ISH
h2ax WT standard conditions Protruding-mouth eye ISH
Protruding-mouth head ISH
slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
Protruding-mouth eye ISH
Protruding-mouth head ISH
hat1 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
hdac1 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
nr2f5 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
ntn1a slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
pcdh10a WT standard conditions Protruding-mouth brain ISH
slbpty77e/ty77e standard conditions Protruding-mouth brain ISH
rx1 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
shha slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
shhb slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
smarcc2 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
tdrd6 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
tfap2a slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
vsx2 slbpty77e/ty77e standard conditions Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
slbpty77e/ty77e standard conditions Long-pec whole organism aldh1a3 expression decreased amount, abnormal
Long-pec whole organism ccnd1 expression increased amount, abnormal
Long-pec whole organism chd7 expression increased amount, abnormal
Long-pec whole organism col10a1a expression decreased amount, abnormal
Long-pec whole organism crabp1b expression increased amount, abnormal
Long-pec whole organism elavl2 expression increased amount, abnormal
Long-pec whole organism gnb3a expression decreased amount, abnormal
Long-pec whole organism h2ax expression increased amount, abnormal
Long-pec whole organism hat1 expression increased amount, abnormal
Long-pec whole organism nr2f5 expression increased amount, abnormal
Long-pec whole organism ntn1a expression increased amount, abnormal
Long-pec whole organism rx1 expression increased amount, abnormal
Long-pec whole organism smarcc2 expression decreased amount, abnormal
Long-pec whole organism tdrd6 expression increased amount, abnormal
Long-pec whole organism tfap2a expression decreased amount, abnormal
Long-pec whole organism vsx2 expression increased amount, abnormal
Protruding-mouth brain pcdh10a expression decreased amount, abnormal
Protruding-mouth eye gnb3a expression decreased amount, abnormal
Protruding-mouth eye gnb3a expression spatial pattern, abnormal
Protruding-mouth eye h2ax expression increased amount, abnormal
Protruding-mouth head h2ax expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One