FIGURE

Fig. 5

ID

ZDB-FIG-190611-31

Publication

Turner et al., 2019 - Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

slbp^ty77e mutant cells fail to transition from proliferation to differentiation.

(A,A’) DNA content of 48 hpf wildtype and slbp^ty77e embryos as assessed by flow cytometry. (B,B’, C,C’) Transverse sections through 48hpf wildtype (B,C) and slbp^ty77emutant (B’,C’) eyes immunostained with anti-BrdU (B,B’) and anti-pH3 antibodies (C,C’). (D,D’) Series of projections of confocal images extracted from live time-lapse movies of tg(EF1α:mAG-zGem(1/100))^rw0410h; tg(EF1α: mKO2-zCdt(1/90))^rw0405b transgenic wildtype (D) and slbp^ty77e mutant (D’) embryos showing cell cycle progression in cells of the forming somites. Green cells are proliferative (S, G2, M) whereas red cells are differentiating somite cells. Scale bar: (B-C’) 50μm; (D-D’) 300μm.

Expression Data

Genes:	mAGFP mKOFP2
Fish:	rw0405bTg; rw0410hTg slbp^ty77e/ty77e; rw0405bTg; rw0410hTg
Anatomical Terms:	somite trunk
Stage Range:	5-9 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slbp^ty77e/ty77e slbp^ty77e/ty77e; rw0405bTg; rw0410hTg
Observed In:	G1 to G0 transition involved in cell differentiation retina cell population proliferation
Stage Range:	10-13 somites to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One