FIGURE

Fig. 4

ID

ZDB-FIG-190611-30

Publication

Turner et al., 2019 - Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits

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Fig. 4

Suppressing cell death partially restores axonal/neuropil deficits in the tectum of slbp^ty77e mutants.

(A-B’) Lateral view of 26hpf wild type (A,B) and slbp^ty77e (A’,B’) heads and eyes stained with TUNEL. Apoptosis is prominent in the tectum (arrowhead) and dorsal hindbrain of the mutant (A’) but not within the eye (B’). The white lines outline the lens within which there is apoptosis in both the wildtype and mutant eyes. (C-C”) Lateral view of 72hpf untreated wildtpe (C), untreated slbp^ty77e (C’) and (C”) caspase inhibitor treated slbp^ty77e heads labelled with anti-acetylated tubulin (axons, green) and anti-SV2 (neuropil, red). Arrow shows aberrant retinal ganglion axons in the mutant eye. Abbreviations: AC, anterior commisure; E, eye; OT, optic tectum; T, telencephalon. Scale bar: (A-A’,C-C”) 100μm; (B-B’) 50μm.

Expression Data

Antibodies:	Ab-SV2 Ab1-tuba
Fish:	WT slbp^ty77e/ty77e
Anatomical Terms:	eye axon eye neuron projection head axon
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slbp^ty77e/ty77e
Condition:	chemical treatment by environment: Z-Val-Ala-Asp(OMe)-CH2F
Observed In:	eye axon eye neuron eye neuron projection hindbrain apoptotic process optic tectum apoptotic process optic tectum neuron optic tectum neuron projection
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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