ZFIN Figure: Harjula et al., 2018, text only

ZFIN ID: ZDB-FIG-190314-11

ZFIN ID: ZDB-FIG-190314-11

Harjula et al., 2018 - Interleukin 10 mutant zebrafish have an enhanced interferon gamma response and improved survival against a Mycobacterium marinum infection. Scientific Reports 8:10360 Full text @ Sci. Rep.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	cd4-1 cd8a foxp3a gata3 ifng1 ighm il1b il4 il10 tbx21 tnfa tnfb
Fish:	WT il10^e46/e46
Condition:	bacterial treatment by injection: Mycobacterium marinum
Anatomical Terms:	kidney pleuroperitoneal region spleen
Stage:	Adult

PHENOTYPE:

Fish:	WT il10^e46/e46
Condition:	bacterial treatment by injection: Mycobacterium marinum
Observed In:	kidney kidney lymphocyte kidney myeloid cell regulation of chronic inflammatory response
Stage:	Adult

Unillustrated author statements

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
cd4-1	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
cd8a	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
foxp3a	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
gata3	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
ifng1	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
ighm	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
il1b	WT	control	Adult		pleuroperitoneal region	RTPCR
	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	control	Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
il4	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
il10	WT	standard conditions	Adult	Not Detected	liver	RTPCR
			Adult		pleuroperitoneal region	RTPCR
			Adult		spleen	RTPCR
	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	standard conditions	Adult	Not Detected	liver	RTPCR
			Adult		pleuroperitoneal region	RTPCR
			Adult		spleen	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
tbx21	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
tnfa	WT	control	Adult		pleuroperitoneal region	RTPCR
	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	control	Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
tnfb	WT	control	Adult		pleuroperitoneal region	RTPCR
	WT	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	control	Adult		pleuroperitoneal region	RTPCR
	il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult		kidney	RTPCR
			Adult		pleuroperitoneal region	RTPCR

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT	bacterial treatment by injection: Mycobacterium marinum	Adult	kidney lymphocyte normal amount, normal
		Adult	kidney myeloid cell normal amount, normal
il10^e46/e46	standard conditions	Adult	kidney lymphocyte normal amount, normal
		Adult	kidney myeloid cell normal amount, normal
		Adult	regulation of chronic inflammatory response process quality, normal
il10^e46/e46	bacterial treatment by injection: Mycobacterium marinum	Adult	kidney cd4-1 expression increased amount, abnormal
		Adult	kidney gata3 expression decreased amount, abnormal
		Adult	kidney ifng1 expression increased amount, abnormal
		Adult	kidney il10 expression increased amount, abnormal
		Adult	kidney il1b expression decreased amount, abnormal
		Adult	kidney tnfb expression decreased amount, abnormal
		Adult	kidney lymphocyte normal amount, normal
		Adult	kidney myeloid cell increased amount, abnormal

Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.