FIGURE

Fig. 4

ID

ZDB-FIG-180817-7

Publication

Mahony et al., 2018 - Oncostatin M and Kit-Ligand Control Hematopoietic Stem Cell Fate during Zebrafish Embryogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

osmr Signaling Is Necessary for HSC Specification

(A) gata2b, runx1, and cmyb ISH in control MO, or osmr MO-injected embryos (injected at 4 ng throughout, unless stated). Scale bar, 100 μm. ctl, control.

(B) ISH expression of cmyb, in control MO, or osmr MO-injected embryos. Scale bar, 100 μm.

(C) rag1 expression in the thymus after control MO or osmr MO injection, along with quantification of thymus area. Data are box and whiskers, min to max. Scale bar, 50 μm.

(D) gata2b, runx1, and cmyb ISH in control MO, or osm MO (injected at 7 ng throughout, unless stated)-injected embryos. Scale bar, 100 μm. Data are boxes and whiskers, min to max. Statistical analysis was completed using an unpaired, two-tailed Student's t test. ^∗∗∗∗p < 0.0001.

Expression Data

Genes:	gata2b myb rag1 runx1
Fish:	AB AB + MO1-osm AB + MO1-osmr
Knockdown Reagents:	MO1-osm MO1-osmr
Anatomical Terms:	caudal hematopoietic tissue dorsal aorta thymus
Stage Range:	26+ somites to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-osm AB + MO1-osmr
Knockdown Reagents:	MO1-osm MO1-osmr
Observed In:	caudal hematopoietic tissue dorsal aorta thymus
Stage Range:	26+ somites to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Stem Cell Reports