ZFIN ID: ZDB-FIG-170505-9
Hasegawa et al., 2017 - Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Pec-fin

Fig. 5

Prolonged il1b expression and apoptosis are induced by macrophage loss during fin fold regeneration.

(A) ISH analysis of il1b expression in spi1b, csf3r, and irf8 morphants. The spi1b and irf8 morphants displayed prolonged il1b expression at 6 hpa. Scale bar, 50 μm. (B) Quantification of il1b expression detected using ISH analysis in (A). The level of il1b expression was evaluated as in Figure 1D. Data are the sum of two experiments (total n > 16 for each MO). (C) TUNEL analysis of the amputated fin fold at 12 hpa after il1b knockdown or Dex treatment in larvae in which macrophages were depleted using the spi1b or irf8 MO. Scale bar, 100 μm. Apoptosis caused by spi1b and irf8 MOs was rescued by il1b MO administration or Dex treatment. (D) Quantification of TUNEL staining in the spi1b morphants (C; bracketed areas). (E) Quantification of TUNEL staining in the irf8 morphants in (C). In (D) and (E), data are presented as means ± SEM. Student’s t test, ***p<0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
il1b WT amputation: caudal fin Long-pec caudal fin regenerating fin ISH
WT + MO1-irf8 amputation: caudal fin Long-pec caudal fin regenerating fin ISH
WT + MO1-spi1b amputation: caudal fin Long-pec caudal fin regenerating fin ISH
WT + MO4-csf3r amputation: caudal fin Long-pec caudal fin regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-il1b + MO1-irf8 amputation: caudal fin Pec-fin regenerating fin apoptotic process occurrence, ameliorated
WT + MO1-il1b + MO1-spi1b amputation: caudal fin Pec-fin regenerating fin apoptotic process occurrence, ameliorated
WT + MO1-irf8 amputation: caudal fin Long-pec caudal fin regenerating fin il1b expression increased amount, abnormal
Pec-fin regenerating fin apoptotic process increased occurrence, abnormal
WT + MO1-irf8 chemical treatment by environment: dexamethasone, amputation: caudal fin Pec-fin regenerating fin apoptotic process occurrence, ameliorated
WT + MO1-spi1b amputation: caudal fin Long-pec caudal fin regenerating fin il1b expression increased amount, abnormal
Pec-fin regenerating fin apoptotic process increased occurrence, abnormal
WT + MO1-spi1b chemical treatment by environment: dexamethasone, amputation: caudal fin Pec-fin regenerating fin apoptotic process occurrence, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife