FIGURE

Fig. 4

ID

ZDB-FIG-170505-7

Publication

Hasegawa et al., 2017 - Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold

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Fig. 4

Macrophages are responsible for survival of regenerative cells.

(A) ISH analysis of lcp1 expression, a macrophage marker, during fin fold regeneration in WT. Scale bar, 50 μm. (B) ISH analysis of mpx expression, a neutrophil marker, during fin fold regeneration in WT. Scale bar, 50 μm. (C) TUNEL analysis in the amputated fin fold at 12 hpa after injection of spi1b, csf3r, and irf8 MOs. Injection of spi1b MO and irf8 MO markedly increased apoptosis of regenerative cells. Scale bar, 100 μm. (D) Quantification of TUNEL-positive cells in the areas bracketed in (A). In (B), data are presented as means ± SEM. Student’s t test, ***p<0.001; N.S., not significant (p=0.981).

Expression Data

Genes:	lcp1 mpx
Fish:	WT
Condition:	amputation: caudal fin
Anatomical Terms:	regenerating fin macrophage regenerating fin neutrophil
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-csf3r WT + MO1-irf8 WT + MO1-spi1b WT + MO4-csf3r
Condition:	amputation: caudal fin
Knockdown Reagents:	MO1-csf3r MO1-irf8 MO1-spi1b MO4-csf3r
Observed In:	macrophage neutrophil regenerating fin apoptotic process
Stage Range:	Long-pec to Pec-fin

Phenotype Detail

Acknowledgments

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