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ZFIN ID: ZDB-FIG-170505-7
Hasegawa et al., 2017 - Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Pec-fin

Fig. 4

Macrophages are responsible for survival of regenerative cells.

(A) ISH analysis of lcp1 expression, a macrophage marker, during fin fold regeneration in WT. Scale bar, 50 μm. (B) ISH analysis of mpx expression, a neutrophil marker, during fin fold regeneration in WT. Scale bar, 50 μm. (C) TUNEL analysis in the amputated fin fold at 12 hpa after injection of spi1b, csf3r, and irf8 MOs. Injection of spi1b MO and irf8 MO markedly increased apoptosis of regenerative cells. Scale bar, 100 μm. (D) Quantification of TUNEL-positive cells in the areas bracketed in (A). In (B), data are presented as means ± SEM. Student’s t test, ***p<0.001; N.S., not significant (p=0.981).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lcp1 WT amputation: caudal fin Long-pec regenerating fin macrophage ISH
Pec-fin regenerating fin macrophage ISH
mpx WT amputation: caudal fin Long-pec regenerating fin neutrophil ISH
Pec-fin regenerating fin neutrophil ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT amputation: caudal fin Long-pec macrophage decreased amount, abnormal
Long-pec neutrophil decreased amount, abnormal
Pec-fin macrophage decreased amount, abnormal
Pec-fin neutrophil decreased amount, abnormal
WT + MO1-csf3r amputation: caudal fin Pec-fin neutrophil decreased amount, abnormal
Pec-fin regenerating fin apoptotic process occurrence, normal
WT + MO1-irf8 amputation: caudal fin Pec-fin macrophage decreased amount, abnormal
Pec-fin regenerating fin apoptotic process increased occurrence, abnormal
WT + MO1-spi1b amputation: caudal fin Pec-fin macrophage decreased amount, abnormal
Pec-fin neutrophil decreased amount, abnormal
Pec-fin regenerating fin apoptotic process increased occurrence, abnormal
WT + MO4-csf3r amputation: caudal fin Pec-fin neutrophil decreased amount, abnormal
Pec-fin regenerating fin apoptotic process occurrence, normal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife