Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-170505-5
Hasegawa et al., 2017 - Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Pec-fin

Fig. 3

il1b knockdown or Dex treatment rescued the clo from apoptosis.

(A) Simultaneous detection of apoptosis by TdT-mediated dUTP nick end labeling (TUNEL) and the il1b-expressing cell at 12 hpa in the clo mutant carrying the transgene il1b:egfp. Most of the TUNEL-positive cells were detected in close association with the il1b-expressing epithelial cells. Vertical and horizontal lines indicate the approximate sites of the optical sections. Scale bar, 100 μm. (B) RT-PCR analysis of il1b and actinb1 expression in zebrafish larvae injected with il1b MO. The il1b MO was targeted to the splice donor site. Arrowhead indicates the aberrant transcript. (C) TUNEL analysis in amputated WT or clo larvae (12 hpa) after il1b knockdown. Injection of il1b MO substantially reduced the apoptosis of regenerative cells in the clo mutant, whereas the apoptosis was unaffected by std MO. Scale bars, 50 μm. (D) Quantification of TUNEL staining in the areas bracketed in (C). (E) ISH analysis of il1b expression at 3 hpa in larvae treated with Dex. DMSO: dimethyl sulfoxide, used as the vehicle. Dex treatment abolished il1b expression. Scale bar, 50 μm. (F) TUNEL staining in the amputated fin fold at 12 hpa in Dex-treated WT and clo larvae. Scale bars, 50 μm. (G) Quantification of TUNEL staining in the areas bracketed in (F). In (D) and (G), data are presented as means ± SEM. Student’s t test, **p<0.01; ***p<0.001; N.S., not significant (p=0.172).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP tyt205Tg ; npas4lm39/m39 amputation: caudal fin Pec-fin caudal fin epithelial cell IHC
Pec-fin regenerating fin epithelial cell IHC
il1b npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec caudal fin regenerating fin ISH
npas4lDf(Chr13:lycat)m39/m39 chemical treatment by environment: dexamethasone, amputation: caudal fin Long-pec caudal fin regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec caudal fin regenerating fin il1b expression increased amount, abnormal
Long-pec regenerating fin apoptotic process increased occurrence, abnormal
Pec-fin regenerating fin apoptotic process increased occurrence, abnormal
npas4lDf(Chr13:lycat)m39/m39 chemical treatment by environment: dexamethasone, amputation: caudal fin Long-pec caudal fin regenerating fin il1b expression amount, ameliorated
Long-pec regenerating fin apoptotic process occurrence, ameliorated
npas4lDf(Chr13:lycat)m39/m39 + MO1-il1b amputation: caudal fin Pec-fin regenerating fin apoptotic process occurrence, ameliorated
tyt205Tg ; npas4lm39/m39 amputation: caudal fin Pec-fin regenerating fin apoptotic process increased occurrence, abnormal
Pec-fin regenerating fin mesenchyme apoptotic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife