ZFIN ID: ZDB-FIG-170505-12
Hasegawa et al., 2017 - Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 7

Il1b-mediated inflammation is required for normal regeneration.

(A) Fin fold regeneration in WT larvae treated with Dex. Regeneration was retarded following treatment with Dex but not vehicle DMSO (arrowheads). Scale bar, 100 μm. (B) Quantification for (A) (posterior to the notochord). (C) BrdU incorporation in larvae treated with DMSO or Dex. BrdU labeling, 0–24 hpa. Scale bar, 50 μm. (D) Quantification for (C) (bracketed areas). BrdU-positive cells were significantly reduced following Dex treatment. (E) Detection of junba expression using ISH and fgf20a expression using the HGn21A enhancer-trap line (Shibata et al., 2016) in DMSO- or Dex-treated larvae. The expression of junba and fgf20a was also significantly downregulated following Dex treatment. Arrowheads indicate EGFP expression at amputation sites. Scale bars, 50 μm (F) Fin fold regeneration in larvae injected with std or il1b MO. The knockdown of il1b reduced fin fold regeneration. Scale bar, 100 μm. (G) Quantification for (F) (posterior to the notochord). (H) BrdU incorporation in larvae injected with std or il1b MO. BrdU labeling, 0–24 hpa. Scale bar, 50 μm. (I) Quantification for (H) (bracketed areas). (J) Detection of junba expression using ISH and fgf20a expression using the HGn21A line in larvae injected with std or il1b MO. The expression of junba and fgf20a was downregulated following il1b knockdown as in the Dex-treated larvae. Arrowheads indicate EGFP expression atamputation sites. Scale bars, 50 μm. (K) Schematic of the procedure of il1b overexpression in uninjured WT larvae. Heat shock was applied twice before fixation. (L) ISH analysis of fn1b, junba, and junbb in WT and Tg(hsp70l:il1b). il1b overexpression stimulated ectopic expression of regeneration-induced genes in uninjured larvae. Scale bar, 50 μm. In (A) and (F), dotted lines indicate amputation planes and fin fold outlines. In (B), (D), (G), and (I), data are presented as means ± SEM. Student’s t test, **p<0.01; ***p<0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP nkhgn21aEt amputation: caudal fin Pec-fin caudal fin regenerating fin IHC
nkhgn21aEt chemical treatment by environment: dexamethasone, amputation: caudal fin Pec-fin caudal fin regenerating fin IHC
nkhgn21aEt + MO1-il1b amputation: caudal fin Pec-fin caudal fin regenerating fin IHC
fn1b WT control Pec-fin caudal fin ISH
tyt204Tg heat shock Pec-fin caudal fin ISH
Pec-fin whole organism ISH
junba WT control Pec-fin Not Detected caudal fin ISH
WT amputation: caudal fin Long-pec caudal fin regenerating fin ISH
Pec-fin caudal fin regenerating fin ISH
WT chemical treatment by environment: dexamethasone, amputation: caudal fin Long-pec caudal fin regenerating fin ISH
Pec-fin caudal fin regenerating fin ISH
WT + MO1-il1b amputation: caudal fin Long-pec caudal fin regenerating fin ISH
Pec-fin caudal fin regenerating fin ISH
tyt204Tg heat shock Pec-fin caudal fin ISH
Pec-fin whole organism ISH
junbb WT control Pec-fin Not Detected caudal fin ISH
tyt204Tg heat shock Pec-fin caudal fin ISH
Pec-fin whole organism ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: dexamethasone, amputation: caudal fin Long-pec caudal fin regenerating fin junba expression decreased amount, abnormal
Pec-fin caudal fin regenerating fin junba expression decreased amount, abnormal
Protruding-mouth regenerating fin cell population proliferation decreased occurrence, abnormal
Day 5 fin regeneration decreased efficacy, abnormal
Day 5 regenerating fin decreased length, abnormal
WT + MO1-il1b amputation: caudal fin Long-pec caudal fin regenerating fin junba expression decreased amount, abnormal
Pec-fin caudal fin regenerating fin junba expression decreased amount, abnormal
Protruding-mouth regenerating fin cell population proliferation decreased occurrence, abnormal
Day 5 fin regeneration decreased efficacy, abnormal
Day 5 regenerating fin decreased length, abnormal
nkhgn21aEt chemical treatment by environment: dexamethasone, amputation: caudal fin Pec-fin caudal fin regenerating fin EGFP expression decreased amount, abnormal
nkhgn21aEt + MO1-il1b amputation: caudal fin Pec-fin caudal fin regenerating fin EGFP expression decreased amount, abnormal
tyt204Tg heat shock Pec-fin caudal fin fn1b expression mislocalised, abnormal
Pec-fin caudal fin junba expression mislocalised, abnormal
Pec-fin caudal fin junbb expression mislocalised, abnormal
Pec-fin whole organism fn1b expression mislocalised, abnormal
Pec-fin whole organism junba expression mislocalised, abnormal
Pec-fin whole organism junbb expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife