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ZFIN ID: ZDB-FIG-170505-3
Hasegawa et al., 2017 - Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 2

Spatiotemporal expression of il1b during fin fold regeneration.

(A) Tissue cryosection of an amputated clo fin fold in which il1b expression was detected using ISH analysis. Arrowheads indicate the epidermal cells expressing il1b. Scale bar, 10 μm. (B) Live imaging of il1b expression in the Tg(il1b:egfp) line in WT and clo mutant at corresponding stages of regeneration. As in the case of il1b expression observed in ISH analysis (Figure 1C), EGFP fluorescence of the Tg(il1b:EGFP) was strongly upregulated in the clo mutant. The EGFP fluorescence in WT decreased more slowly than did the il1b mRNA signal, because of the comparatively longer half-life of the protein EGFP. Scale bars, 100 μm. (C) Immunofluorescence detection of EGFP and E-cadherin in Tg(il1b:EGFP) at 3 and 12 hpa. Confocal longitudinal and transverse optical sections are shown at the top and right side, respectively. Vertical and horizontal red and green lines indicate the approximate sites of the optical sections. Scale bars, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP tyt205Tg control Long-pec caudal fin IFL
tyt205Tg amputation: caudal fin Long-pec caudal fin epithelial cell IHC
Long-pec caudal fin regenerating fin IFL
Long-pec regenerating fin epithelial cell IHC
Pec-fin caudal fin epithelial cell IHC
Pec-fin caudal fin regenerating fin IFL
Pec-fin regenerating fin epithelial cell IHC
Protruding-mouth caudal fin regenerating fin IFL
Day 4 Not Detected caudal fin regenerating fin IFL
tyt205Tg ; npas4lm39/m39 control Long-pec caudal fin IFL
tyt205Tg ; npas4lm39/m39 amputation: caudal fin Long-pec caudal fin epithelial cell IHC
Long-pec caudal fin regenerating fin IFL
Long-pec regenerating fin epithelial cell IHC
Pec-fin caudal fin epithelial cell IHC
Pec-fin caudal fin regenerating fin IFL
Pec-fin regenerating fin epithelial cell IHC
Protruding-mouth caudal fin regenerating fin IFL
Day 4 caudal fin regenerating fin IFL
il1b npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec caudal fin epidermal cell ISH
Long-pec regenerating fin epidermal cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tyt205Tg ; npas4lm39/m39 amputation: caudal fin Long-pec caudal fin regenerating fin EGFP expression increased amount, abnormal
Pec-fin caudal fin regenerating fin EGFP expression increased amount, abnormal
Protruding-mouth caudal fin regenerating fin EGFP expression increased amount, abnormal
Day 4 caudal fin regenerating fin EGFP expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife