Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-170505-2
Hasegawa et al., 2017 - Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Long-pec

Fig. 1

Prolonged activation of il1b expression in the clo mutant after fin fold amputation.

(A) Transcriptome analysis of posterior fin fold tissues from uncut WT, amputated WT, and amputated clo mutant at 6 hpa. The ordinate, final RPKM, indicates the reads per kilobase of exon model per million mapped reads. Expression of regeneration-induced genes such as fn1b, junba, junbb, mmp9, and fgf20a was upregulated in the clo mutant. Notably, expression of inflammation-related genes such as il1b, tnf1b, and ptgs2a was highly upregulated in the amputated clo mutant. (B) RT-PCR analysis of il1b expression in WT and clo larvae at 3, 6, and 12 hpa. actb1: actinb1. (C) ISH analysis of il1b expression at corresponding stages of regeneration in WT and clo. Arrowheads indicate myeloid-like cells expressing il1b. Scale bars, 25 μm. (D) Quantification of il1b expression. The level of il1b expression was divided into four groups according to the ISH signal; the numbers in the bars indicate the ratios of the respective groups. Data are the sum of three experiments (total n > 13 for each sample). The data suggest that il1b expression extended into later stages in clo than in WT. (E) ISH analysis of il1b expression after LPS injection. Phenol red: negative control containing only phenol red; hpi: hours post injection. Scale bars, 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fgf20a npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec whole organism posterior region RTPCR
fn1b npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec whole organism posterior region RTPCR
il1b WT chemical treatment by injection: lipopolysaccharide Long-pec myeloid cell ISH
WT amputation: caudal fin Long-pec caudal fin myeloid cell ISH
Long-pec caudal fin regenerating fin ISH
Long-pec whole organism posterior region RTPCR
Pec-fin Not Detected caudal fin regenerating fin ISH
Pec-fin Not Detected whole organism posterior region RTPCR
Protruding-mouth Not Detected caudal fin regenerating fin ISH
npas4lDf(Chr13:lycat)m39/m39 chemical treatment by injection: lipopolysaccharide Long-pec myeloid cell ISH
npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec caudal fin regenerating fin ISH
Long-pec whole organism posterior region RTPCR
Pec-fin Not Detected caudal fin regenerating fin ISH
Pec-fin Not Detected whole organism posterior region RTPCR
Protruding-mouth Not Detected caudal fin regenerating fin ISH
junba npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec whole organism posterior region RTPCR
junbb npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec whole organism posterior region RTPCR
mmp9 npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec whole organism posterior region RTPCR
ptgs2a npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec whole organism posterior region RTPCR
tnfb npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec whole organism posterior region RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
npas4lDf(Chr13:lycat)m39/m39 chemical treatment by injection: lipopolysaccharide Long-pec myeloid cell decreased amount, abnormal
npas4lDf(Chr13:lycat)m39/m39 amputation: caudal fin Long-pec caudal fin regenerating fin il1b expression increased amount, abnormal
Long-pec whole organism posterior region fgf20a expression increased amount, abnormal
Long-pec whole organism posterior region fn1b expression increased amount, abnormal
Long-pec whole organism posterior region il1b expression increased amount, abnormal
Long-pec whole organism posterior region junba expression increased amount, abnormal
Long-pec whole organism posterior region junbb expression increased amount, abnormal
Long-pec whole organism posterior region mmp9 expression increased amount, abnormal
Long-pec whole organism posterior region ptgs2a expression increased amount, abnormal
Long-pec whole organism posterior region tnfb expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife