ZFIN ID: ZDB-FIG-140919-10
Pi-Roig et al., 2014 - Distinct tissue-specific requirements for the zebrafish tbx5 genes during heart, retina and pectoral fin development. Open Biology   4:140014 Full text @ Open Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5

tbx5b knock-down causes a delay in pectoral fin growth. (a,i,q) Pectoral fin morphology at 3 dpf. Dorsal views are shown with anterior to the top. (b-h) Expression of the developing pectoral fin markers in control (ctrl) MO-injected embryos. (c′-h′) Higher magnifications of (c-h). (j-p) Pectoral fin markers expression in tbx5b-morphant embryos. (k′-p′) Higher magnifications of (k-p). (r,s) tbx5a morphants. (t) Model for the differential requirements for the tbx5 genes during pectoral fin development. b-h, j-p,r,s are dorsal views with anterior to the left. c′-h′,k′-p′ are lateral views with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etv4 WT control Prim-25 mesenchyme pectoral fin ISH
Prim-25 pectoral fin bud ectoderm ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ectoderm ISH
WT + MO1-tbx5b standard conditions Prim-25 mesenchyme pectoral fin ISH
Prim-25 Not Detected pectoral fin bud ectoderm ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ectoderm ISH
fgf10a WT control Prim-5 mesoderm pectoral fin bud ISH
WT + MO1-tbx5b standard conditions Prim-5 mesoderm pectoral fin bud ISH
fgf24 WT control Prim-5 mesoderm pectoral fin bud ISH
Prim-25 mesenchyme pectoral fin ISH
Prim-25 pectoral fin bud ectoderm ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ectoderm ISH
WT + MO1-tbx5b standard conditions Prim-5 mesoderm pectoral fin bud ISH
Prim-25 mesenchyme pectoral fin ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ectoderm ISH
tbx5a WT control Prim-5 mesoderm pectoral fin bud ISH
WT + MO1-tbx5b standard conditions Prim-5 mesoderm pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Protruding-mouth pectoral fin aplastic, abnormal
Protruding-mouth pectoral fin development disrupted, abnormal
WT + MO1-tbx5b standard conditions Protruding-mouth pectoral fin development delayed, abnormal
Protruding-mouth pectoral fin immature, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Open Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Open Biol.