ZFIN ID: ZDB-FIG-140919-10
Pi-Roig et al., 2014 - Distinct tissue-specific requirements for the zebrafish tbx5 genes during heart, retina and pectoral fin development. Open Biology   4:140014 Full text @ Open Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5

tbx5b knock-down causes a delay in pectoral fin growth. (a,i,q) Pectoral fin morphology at 3 dpf. Dorsal views are shown with anterior to the top. (b-h) Expression of the developing pectoral fin markers in control (ctrl) MO-injected embryos. (c′-h′) Higher magnifications of (c-h). (j-p) Pectoral fin markers expression in tbx5b-morphant embryos. (k′-p′) Higher magnifications of (k-p). (r,s) tbx5a morphants. (t) Model for the differential requirements for the tbx5 genes during pectoral fin development. b-h, j-p,r,s are dorsal views with anterior to the left. c′-h′,k′-p′ are lateral views with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etv4 WT control Prim-25 mesenchyme pectoral fin ISH
Prim-25 pectoral fin bud ectoderm ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ectoderm ISH
WT + MO1-tbx5b standard conditions Prim-25 mesenchyme pectoral fin ISH
Prim-25 Not Detected pectoral fin bud ectoderm ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ectoderm ISH
fgf10a WT control Prim-5 mesoderm pectoral fin bud ISH
WT + MO1-tbx5b standard conditions Prim-5 mesoderm pectoral fin bud ISH
fgf24 WT control Prim-5 mesoderm pectoral fin bud ISH
Prim-25 mesenchyme pectoral fin ISH
Prim-25 pectoral fin bud ectoderm ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ectoderm ISH
WT + MO1-tbx5b standard conditions Prim-5 mesoderm pectoral fin bud ISH
Prim-25 mesenchyme pectoral fin ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ectoderm ISH
tbx5a WT control Prim-5 mesoderm pectoral fin bud ISH
WT + MO1-tbx5b standard conditions Prim-5 mesoderm pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Protruding-mouth pectoral fin aplastic, abnormal
Protruding-mouth pectoral fin development disrupted, abnormal
WT + MO1-tbx5b standard conditions Protruding-mouth pectoral fin development delayed, abnormal
Protruding-mouth pectoral fin immature, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Open Biol.