ZFIN ID: ZDB-FIG-140919-2
Pi-Roig et al., 2014 - Distinct tissue-specific requirements for the zebrafish tbx5 genes during heart, retina and pectoral fin development. Open Biology   4:140014 Full text @ Open Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2

tbx5 morphants exhibit cardiac jogging and lefty2 expression defects. (a-a′′) tbx5a morphant phenotypes. (b-b′′) tbx5b morphants. (c-e′′) Left, right and middle jog phenotypes obtained by co-injection of tbx5a and tbx5b MOs at 0.5 ng (c-c′′), 1.5 ng (d-d′′) or 3 ng (e-e′′) of each MO. (f) Control (ctrl) morphant. (g) Quantification of the phenotypes. (h-j) lefty2 expression in control (h) and double-morphant (i,j) 22-somite stage embryos. (k) Quantification of the phenotypes. A χ2 test has been used to assess significant differences between groups (**p < 0.001). All images are dorsal views with anterior to the top, and myl7 expression is used to highlight the developing heart tube in a-f.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lft2 WT control 20-25 somites heart rudiment ISH
WT + MO1-tbx5a + MO1-tbx5b standard conditions 20-25 somites heart rudiment ISH
myl7 WT control Prim-5 heart tube ISH
WT + MO1-tbx5a standard conditions Prim-5 heart tube ISH
WT + MO1-tbx5a + MO1-tbx5b standard conditions Prim-5 heart tube ISH
WT + MO1-tbx5b standard conditions Prim-5 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Prim-5 heart jogging disrupted, abnormal
WT + MO1-tbx5a + MO1-tbx5b standard conditions Prim-5 heart jogging disrupted, abnormal
WT + MO1-tbx5b standard conditions Prim-5 heart jogging disrupted, abnormal
Acknowledgments:
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