ZFIN ID: ZDB-FIG-140919-9
Pi-Roig et al., 2014 - Distinct tissue-specific requirements for the zebrafish tbx5 genes during heart, retina and pectoral fin development. Open Biology   4:140014 Full text @ Open Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

tbx5 genes are required for dorsoventral retina organization. (a) Schematics of our quantification method. Expression of efn2a and ephB2 in control (ctrl) embryos (b-b′), tbx5a morphants (c-c′) and tbx5b morphants (d-d′). (e-g′) Expression of efn2a and ephB2 in embryos co-injected with different concentrations of both tbx5a and tbx5b MOs. (h-i) Quantification of the results obtained for the expression of efn2a (h) and ephB2 (i). (j-m) Retinal projections of 48 hpf ath5:GFP embryos injected with control, tbx5a, tbx5b or tbx5a and tbx5b MO. (n) Optic nerve diameter quantifications. Data are represented as the mean ± s.e. A Kruskal-Wallis test was used to determine statistical differences among experimental groups (*p < 0.05, **p < 0.001). D, dorsal; N, nasal; T, temporal; V, ventral.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
efnb2a WT control Long-pec retina dorsal region ISH
WT + MO1-tbx5a standard conditions Long-pec retina dorsal region ISH
WT + MO1-tbx5a + MO1-tbx5b standard conditions Long-pec retina dorsal region ISH
WT + MO1-tbx5b standard conditions Long-pec retina dorsal region ISH
ephb2a WT control Long-pec retina ventral region ISH
WT + MO1-tbx5a standard conditions Long-pec retina ventral region ISH
WT + MO1-tbx5a + MO1-tbx5b standard conditions Long-pec retina ventral region ISH
WT + MO1-tbx5b standard conditions Long-pec retina ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a + MO1-tbx5b standard conditions Long-pec cranial nerve II decreased thickness, abnormal
Long-pec retinal ganglion cell axon guidance process quality, normal
Acknowledgments:
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