ZFIN ID: ZDB-FIG-140919-1
Pi-Roig et al., 2014 - Distinct tissue-specific requirements for the zebrafish tbx5 genes during heart, retina and pectoral fin development. Open Biology   4:140014 Full text @ Open Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 1

Knock-down of tbx5 genes causes cardiac looping defects. (a-c) Embryos injected with control MO or sub-optimal concentrations of tbx5a or tbx5b MOs. (d-d′′) tbx5a morphant phenotypes. (e-e′) tbx5b-morphant phenotypes. (f-h′′) Double knock-down of tbx5 genes (0.5 ng each MO (f-f′′), 1.5 ng each MO (g-g′′) and 3 ng each MO (h-h′′)). (i) Quantification of the degree of looping phenotypes: wt, complete; phenotype, incomplete looping s.o., sub-otimal. (j) Quantification of the looping orientation phenotypes. A χ2 statistic has been calculated to assess significant differences between groups (**p < 0.001, *p < 0.05). Images are frontal views of 48 hpf embryos, and myl7 expression is used to highlight the developing heart.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Long-pec heart looping arrested, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec heart malformed, abnormal
WT + MO1-tbx5a + MO1-tbx5b standard conditions Long-pec heart looping arrested, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec heart malformed, abnormal
WT + MO1-tbx5b standard conditions Long-pec heart looping arrested, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec heart malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Open Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Open Biol.