ZFIN ID: ZDB-FIG-131231-40
van der Velden et al., 2013 - The polycomb group protein ring1b/rnf2 is specifically required for craniofacial development. PLoS One   8(9):e73997 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 4 Cranial neural crest cells migrate into the pharyngeal arches of ring1b mutants.

Dorsal (A–H) and lateral (A′–H′) views of WT and ring1b mutants at 32 hpf stained with the indicated genes. Pre-chondrogenic gene expression in CNCs is largely unaffected in ring1b mutants. Dlx2a is expressed at normal levels in the pharyngeal arches of ring1b mutants (A–B′). Separation of dlx2a-positive CNCs into distinct groups of cells that populate the posterior arches is slightly affected in ring1b mutants A–B′). Hand2 expression in the ring1b pharyngeal arches is identical to that of WT siblings (C–D′). Hoxa3a expression is reduced in the ring1b posterior pharyngeal arches (E–F′). Expression of the pharyngeal pouch marker cyp26a1 is severely reduced in ring1b mutants (G–H′).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cyp26a1 WT standard conditions Prim-15 pharyngeal pouch ISH
rnf2ibl30/ibl30 standard conditions Prim-15 pharyngeal pouch ISH
rnf2ibl31/ibl31 standard conditions Prim-15 pharyngeal pouch ISH
dlx2a WT standard conditions Prim-15 pharyngeal arch cranial neural crest cell ISH
rnf2ibl30/ibl30 standard conditions Prim-15 pharyngeal arch cranial neural crest cell ISH
rnf2ibl31/ibl31 standard conditions Prim-15 pharyngeal arch cranial neural crest cell ISH
hand2 WT standard conditions Prim-15 pharyngeal arch cranial neural crest cell ISH
rnf2ibl30/ibl30 standard conditions Prim-15 pharyngeal arch cranial neural crest cell ISH
rnf2ibl31/ibl31 standard conditions Prim-15 pharyngeal arch cranial neural crest cell ISH
hoxa3a WT standard conditions Prim-15 pharyngeal arch 3-7 ISH
rnf2ibl30/ibl30 standard conditions Prim-15 pharyngeal arch 3-7 ISH
rnf2ibl31/ibl31 standard conditions Prim-15 pharyngeal arch 3-7 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rnf2ibl30/ibl30 standard conditions Prim-15 pharyngeal pouch cellular quality, abnormal
rnf2ibl31/ibl31 standard conditions Prim-15 pharyngeal pouch cellular quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One